汉坦病毒基因重排病毒株毒力变化的初步研究  

The study of the virulence of genetic reassortment viruses of Hantaviruses

作　　者：康文臻[1] 黄长形[1] 白雪帆[1] 杨为松[1] 李光玉[1] 郝春秋[1] 谢玉梅[1] 

机构地区：[1]第四军医大学唐都医院感染病科,陕西西安710038

出　　处：《医学研究生学报》2002年第4期290-291,297,共3页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助课题 (批准号 :396 70 6 5 0 )

摘　　要：目的 :比较汉坦病毒基因重排病毒L7MSS7和L7MSS7S株与亲代病毒 76 118株、SR 11株毒力的差异。　方法 :选取汉坦病毒基因重排病毒株L7MSS7株和L7MSS7S株 ,分别接种于VeroE6细胞和乳小鼠 ,观察其在VeroE6细胞中的增殖特性和对乳小鼠致死性 ,并与亲代病毒 76 118株、SR 11株进行比较。　结果 :发现L7MSS7S株在VeroE6细胞中较其他病毒株生长缓慢 ,接种后 14天无一只死亡。L7MSS7株接种于VeroE6细胞中第 8天 ,约 3 5的VeroE6细胞脱落 ,接种后第 10天乳小鼠存活率 75 % ,较亲代病毒显著增高。　结论Objectives:Comparing the virulence and biological characteristic of the genetic reassorment viruses of Hantaviruses with the parental strains. Methods:To study the propagation and replication of genetic reassortment viruses strains of Hantaviruses L 7M SS 7 strains and L 7M SS 7S strains in Vero E6 cells,and the virulence in newborn mice. Results:The propagation and replication of L 7M SS 7S strains was slower than the parental strains in Vero E6 cells,and no newborn mice died after 20 days of inoculation.75% of newbon mice survived after 10 days of inoculation L 7M SS 7 strains,the viability is much higher than the parental strains. Conclusions:There is difference in virulent and biological characteristic between the genetic reassorment viruses and the parental strains.

关 键 词：汉坦病毒 基因重排 毒力 生物学特性 

分 类 号：R373[医药卫生—病原生物学]