枇杷内生木霉P3.9菌株液体发酵条件优化筛选  

Screening on Conditions of Liquid Fermentation for Endotrophic Trichoderma Strain P3.9 from Loquat

作　　者：洪亮[1] 胡金碧 鲁海菊[1] 张海燕[1] 李丽莎[1] 

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661199

出　　处：《湖北农业科学》2017年第14期2670-2674,共5页Hubei Agricultural Sciences

基　　金：云南省应用基础研究计划项目(2016FB066);国家自然科学基金项目(31660147);红河学院应用型科学研究重点项目(XJY15Z06)

摘　　要：为弄清枇杷内生木霉P3.9菌株最佳发酵条件,以分离自枇杷主干韧皮部的内生木霉P3.9菌株为供试菌株,以菌丝生长量为指标,通过单因素和正交试验,筛选最佳组合液体发酵培养液,碳源、氮源、pH及其接种量。结果表明,最佳培养液为马铃薯浸汁,碳源为D-果糖,氮源为磷酸二氢铵,pH 7.0,接种量为1.5%;正交试验表明其菌丝生长的最佳组合培养条件为马铃薯浸汁,在其中添加2%D-果糖、0.05%磷酸二氢铵、pH 6.0、接种量为1.5%。按优化后的培养条件培养,最高菌丝干重可达5.1 mg/m L。In order to make clear optimal conditions of liquid fermentation for Trichoderma strain P3.9,Taking Trichoderma strain P3.9 isolated from trunk phloem of loquat as material,optimal substrates and its concentration of medium,including carbon source,nitrogen source,pH and inoculation count were studied according to the mycelial production by simple factor and orthogonal experiments,in order to screen out the best liquid fermentation for P3.9 strain. The results showed that the proper medium was potato juice; Carbon source was Dfructose; Nitrogen source was ammonium phosphate monobasic; pH was 7.0;Inoculation count was 1.5﹪. The orthogonal experiment indicated that optimal medium of the highest yields of mycelia consisted potato juice,2﹪ D-fructose. 0.05﹪ was ammonium phosphate monobasic. pH was 6.0. Inoculation count was 1.5﹪. By the optimal medium and culture conditions,the maximum mycelial yield was 5.1 mg∕mL.

关 键 词：内生菌 木霉 液体发酵 菌丝干重 

分 类 号：S667.3[农业科学—果树学]