膝眼穴针灸对大鼠膝关节软骨细胞表型以及PGC-1α信号通路的影响  

Effect of acupuncture for the knee point on chondrocyte phenotype and PGC-1α signal pathway in knee

作　　者：苗岩松[1] 徐卿荣[1] 李鹤[1] 姚菊芳[1] 董宇启[1] 

机构地区：[1]上海交通大学医学院附属仁济医院骨科,上海200127

出　　处：《临床骨科杂志》2017年第4期503-507,共5页Journal of Clinical Orthopaedics

摘　　要：目的研究膝眼穴针灸疗法对膝关节软骨细胞表型改变及其参与线粒体生成氧自由基(ROS)的过氧化物酶体增殖物活化受体γ协同刺激因子1α(PGC-1α)信号通路变化的影响。方法通过免疫组化技术检测膝眼穴针灸后大鼠膝关节软骨细胞表型的主要特征分子指标(col 1、col 2、aggrecan)以及PGC-1α、线粒体转录因子A(TFAM)、解耦联蛋白2(UCP 2)和NADPH氧化酶1/4(NOX 1/4)因子的表达变化。进一步检测向大鼠膝关节腔内注射ZN005L(PGC-1α激活剂)激活PGC-1α表达后大鼠软骨细胞表型特征分子指标的表达变化,以及软骨细胞内ROS生成量的改变。结果对大鼠进行膝眼穴针灸后,PGC-1α表达增加;UCP 2以及软骨细胞表型主要指标aggrecan表达下降。激活PGC-1α表达后,软骨细胞内ROS的生成量降低,并且软骨细胞表型丢失现象被抑制。结论膝眼穴针灸疗法不能改善大鼠膝关节软骨细胞的功能,相反会促进软骨细胞表型特征分子的丢失,并损害软骨细胞功能。Objective To study the effects of acupuncture for the knee point on the change of chondrocyte phenotype and the peroxisome proliferators activated receptor gamma co-activator 1 alpha （PGC-la） signaling pathway involved in mitochondrial oxygen free radical （ROS）. Methods The main indicators （col 1 , col 2 , aggrecan） of chondrocyte phenotype of the rat knee cartilage, PGC-la, mitochondrial transcriptional factor A （TFAM） , uncoupling protein 2 （UCP 2） and NADPH oxidasel/4（NOX 1/4） were detected by immunohistochemical technique afer the knee joints of the rats were acupuncture for the knee point. Moreover, after ZN005L was injected into the knee joint of the rat to activate the expression of PGC-la,the changes of chondrocyte phenotype and ROS were detected. Results The up- regulation of PGC-la could inhibit the loss of chondrocyte phenotype and reduced the production of ROS. Although acupuncture for the knee point could increase the expression of PGC-1α, it reduced the mitochondrial function andthe effect of the coupling,and leaded to the loss of chondrocyte phenotype. Conclusions Acupunture therapy can not improve the function of chondrocytes, on the contrary, it can promote the loss of chondrocyte phenotype and destroy the function of the chondrocytes.

关 键 词：膝眼穴针灸 软骨细胞表型 过氧化物酶体增殖物活化受体γ协同刺激因子1α 线粒体转录因子A 解耦联蛋白2 

分 类 号：R684.3[医药卫生—骨科学]