4种自身抗原与狼疮性肾炎相关性的CrELISA分析  

Identification of Four Potential Lupus Nephritis-associated Autoantigens by CrELISA

作　　者：胡桑[1,2] 谭杰文[1,2] 乔玉莉[1,2] 龚祖康 黄渝[1,2] 廖维芳[1,2] 周素芳[1,2] 陈志坚[4] 苏家光[5] 林文珍[1,2] 

机构地区：[1]广西医科大学基础医学院生物化学与分子生物学教研室,广西南宁530021 [2]广西高校生物分子医学研究重点实验室,广西南宁530021 [3]南宁急救医疗中心,广西南宁530001 [4]广西医科大学第一附属医院检验科,广西南宁530021 [5]广西医科大学第一附属医院皮肤性病科,广西南宁530021

出　　处：《系统医学》2016年第6期1-4,共4页Systems Medicine

基　　金：国家自然科学基金项目(81160263);广西自然科学基金项目(2010GXNSFA013162)

摘　　要：目的分析4种自身抗原与狼疮性肾炎(LN)的相关性,以寻找具有更高敏感性与特异性的特征性自身抗原。方法收集广西医科大学第一附属医院检验科2013年7月—2014年7月期间的患者血清120例,其中正常人血清、系统性红斑狼疮(SLE)患者血清、LN患者血清及肾病综合征(NS)患者血清各30例。以Cr ELISA法检测四种阳性抗原蛋白在各组血清中的表达情况,并以生物信息学分析结果。结果 A1、A2在SLE组中的表达量(0.67±0.36)、(0.31±0.18)相对在正常人组中的表达量(0.47±0.24)、(0.22±0.10)与NS组中的表达量(0.33±0.17)、(0.19±0.10)更高(P<0.05),与LN组中的表达量(0.53±0.27)、(0.27±0.14),差异无统计学意义(P>0.05)。结论 4种抗原中,A1、A2的敏感性相对较高,更可能是SLE/LN的特征性自身抗原,有望成为SLE/LN的诊断依据。Objective To assess the relevance of four autoantigens to lupus nephritis（LN）, and find out some more sensitive and specific characteristic autoantigens. Methods Sera were acquired from Laboratory Department of the First Affiliated Hospital of Guangxi Medical University in July 2013 to July 2014, containing 30 normal sera, 30 systemic lupus erythematosus（SLE） patients＇ sera, 30 LN patients＇ sera and 30 nephrotic syndrome（NS） patients＇ sera. The normal group came from healthy examinees. The SLE group was collected according to SLICC Revision of the ACR Classification Criteria for SLE. The LN group came from those SLE patients with renal lesions. The NS group came from NS patients without SLE. Crude lysate enzyme-linked immunosorbent assay（Cr ELISA） was used to detect potential antigens＇ expression levels in each group. Bioinformatics method was used to analyze the results. Results Expression levels of A1 and A2 in SLE group（0.67±0.36）,（0.31±0.18） are statistically higher than those in normal group（0.47±0.24）,（0.22±0.10） and NS group（0.33±0.17）,（0.19±0.10）（P〈0.05）, but similar to those in LN group（0.53±0.27）,（0.27±0.14）（P〉0.05）. Conclusion Among these four autoantigens, A1 and A2 suggest higher sensibility, which make them the more possible characteristic autoantigens of SLE/LN and hopeful diagnostic basis.

关 键 词：狼疮性肾炎 系统性红斑狼疮 自身抗原 CrELISA法 

分 类 号：R593[医药卫生—内科学]