广西地区部分口岸鼠传疾病病原体调查  

Investigation on rodent-borne pathogens at frontier ports in Guangxi Zhuang Autonomous Region

作　　者：叶海波[1,2] 万道正 许佳[1,3] 云小云 张晓龙[1] 覃凤葵 陈健超 方勇 姚李四[1] YE Hai-bo WAN Dao-zheng XU Jia YUN Xiao-yun ZHANG Xiao-long QIN Feng-kui CHEN Jian-ehao FANG Yong YAO Li-si(Chinese Academy of Inspection and Quaranine, Beijing 100176, China Guangxi Entry-Exit Inspection and Quarantine Bureau Anhui International Travel and Health Care Center Hainan International Travel and Health Care Center)

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]广西出入境检验检疫局,南宁530021 [3]安徽国际旅行卫生保健中心,合肥230022 [4]海南国际旅行卫生保健中心,海口571000

出　　处：《中国媒介生物学及控制杂志》2017年第4期343-346,共4页Chinese Journal of Vector Biology and Control

基　　金：国家质检总局公益项目(20140176)~~

摘　　要：目的掌握广西壮族自治区(广西)口岸小兽自然感染汉坦病毒、立克次体、伯氏疏螺旋体、巴尔通体和钩端螺旋体(钩体)的情况。方法于2014-2015年在广西8个口岸辖区范围内采用鼠笼法捕获小兽,取其肺、肝和肾脏组织。从小兽肺中提取RNA,利用RT-PCR扩增汉坦病毒核酸;从小兽肝和肾脏中提取DNA,用PCR方法分别扩增立克次体、伯氏疏螺旋体、巴尔通体和钩体核酸。结果捕获黄胸鼠、褐家鼠和臭鼩鼱共86只,其中褐家鼠数量最多,占捕获总数的67.4%(58/86);汉坦病毒核酸、巴尔通体和钩体总阳性率分别为3.5%(3/86)、2.3%(2/86)和35.3%(6/17);未检测到立克次体和伯氏疏螺旋体核酸。结论广西地区部分口岸小兽可能有汉坦病毒、巴尔通体和钩体自然感染,应加强口岸小兽及其传播疾病的监测。Objective To understand the prevalence of rodent- borne pathogens in the ports of Guangxi Zhuang Autonomous Region. Methods The small mammals were captured by mouse cages in eight ports in Guangxi. The small mammals lung, liver and kidney specimens were obtained by dissection in laboratory. Total RNA was extracted from lung specimens, and Hantavirus nucleotide acid was amplified by RT-PCR. DNA was extracted from liver and kidney specimens. Rickettsia, Borrelia burgdorferi, Bartonella and Leptospira interrogans nucleotide acid were amplified by PCR, respectively. Results A total of 86 small mammals including Rattus tanezumi, R. norvegicus, and Suncus murinus were captured. The positive rate of Hantavirus, Bartonella, and Leptospira interrogans nucleotide acid were 3.5% （3/86）, 2.3% （2/86）, and 35.3% （6/17）; No Rickettsia and B. burgdorferi spirochetes were detected in this study. Conclusion Hantavirus, Bartonella, and Leptospira interrogans might be carried by small mammals in the regions of Guangxi ports. It is necessary to strengthen rodent-borne diseases surveillance in the ports of Guangxi Zhuang Autonomous Region.

关 键 词：小兽 汉坦病毒 立克次体 伯氏疏螺旋体 巴尔通体 钩端螺旋体 

分 类 号：S443[农业科学—农业昆虫与害虫防治] R37[农业科学—植物保护]