雄激素受体基因CAG重复序列多态及雄激素水平与男性盐敏感性高血压相关性的临床研究  

Clinical Study of Androgen Receptor(CAG) Gene Polymorphism and Androgen in Male with Salt Sensitive Hypertension

作　　者：尚亮[1] 卫娜[1] 马庆[1] 刘延梅[1] 张静[1] 时晔[1] 苗青[1] 

机构地区：[1]陕西省人民医院西院二病区,西安710068

出　　处：《现代检验医学杂志》2017年第4期47-49,共3页Journal of Modern Laboratory Medicine

基　　金：陕西省科技厅社发攻关项目(2014K11-03-01-06)

摘　　要：目的研究男性盐敏感性高血压患者雄激素受体基因CAG重复序列多态及雄激素水平的相关性。方法通过口服盐水负荷试验及速尿缩容法将男性高血压组人群分为盐敏感者(SS组)及盐不敏感者(SR组),对61例SS组和40例SR组患者及60例年龄匹配的男性正常对照组采用测序方法分析(CAG)n的重复数,并采用电化学发光法测定血清中总睾酮(TT)、游离睾酮(FT)水平。结果 161例研究对象的CAG重复次数介于14~34次,平均22.4±2.7。SS,SR和健康对照组CAG重复次数分别为23.5±3.75,22.3±3.17和21.8±2.95,三组之间比较差异均有统计学意义(t=2.627~3.257,均P<0.05)。与健康对照组比较,SS,SR两组TT,FT水平下降,且SS组下降更明显,TT水平三组之间比较差异均有统计学意义(t=2.618~3.215,均P<0.05)。FT水平三组之间比较差异均有统计学意义(t=2.524~3.826,均P<0.05)。结论雄激素受体基因CAG重复序列多态及雄激素水平与男性高血压特别是盐敏感性高血压发病有关,长CAG重复序列多态可能是高血压的遗传因素之一,血浆雄激素水平可能作为男性盐敏感性高血压的预测指标。Objective To investigate the＇relationship between androgen receptor CAG gene polymorphism and androgen in male with salt sensitive hypertension. Methods Through the oral saline loading test and furosemide volume method male hypertension group were divided into salt-sensitive （SS group） and salt-insensitive （SR group）. The samples from 161 males were selected in the study, including salt-sensitive hypertension patients （SS group, 61/161）, salt-insensitive hypertension patients （SR group,40/161） and age-matched healthy samples （control group,60/161）. All samples were sequenced with an analysis method （CAG） n repeated polymorphism,and determinated of total testosterone （TT） and free testosterone （FT） level in serum by electrochemiluminescence immunoassay. Results The number of CAG repeats was 14-34,average 22.4- 2.7. The CAG repeats of SS, SR and control group were 23.5±3.75,22.3±3.17 and 21.8±2.95 ,respectively. There were significant differences among the three groups （t= 2. 627- 3. 257, all P〈0.05）. The level of TT and FT in SS and SR group were decreased compared with that of control group. At the same time,the level of SS group was lower,and there were significant differences among the three groups （t= 2. 524±3. 826, all P〈0.05）. Conclusion The androgen receptor gene re- peat length and androgen levels are associated with male hypertension,especially salt-sensitive hypertension. Long （CAG） n repeat polymorphism maybe a genetic factor in the pathogenesis of hypertension. Plasma androgen levels may be used as a predictor of male salt-sensitive hypertension.

关 键 词：盐敏感性高血压 雄激素受体 雄激素 

分 类 号：R544.1[医药卫生—心血管疾病] Q786[医药卫生—内科学]