2015～2016年天津地区新生儿TORCH血清学筛查及感染特点  

TORCH Serological Screening and Infection Characteristic in Neonate in Tianjin Area

作　　者：刘震忠[1] 吴凤琪[1] 闫静[1] 黄艳[1] 刘文康[2] 

机构地区：[1]天津市职业病防治院(工人医院),天津300011 [2]陕西省人民医院检验科,西安710068

出　　处：《现代检验医学杂志》2017年第4期133-136,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探讨天津地区新生儿感染弓形体(Toxoplasma gondii,TOX)、风疹病毒(Rubella virus,RV)、巨细胞病毒(Cytomegalovirus,CMV)和单纯疱疹病毒Ⅱ型(Herpes simplex virusⅡtype,HSV-Ⅱ)(TORCH)状况及特点。方法利用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测2015~2016年2 273例出生后28天内新生儿血清中TOX-IgM/AgG,RV-IgM/AgG,CMV-IgM/IgG和HSV-Ⅱ-IgM/IgG。结果在2 273例新生儿中TOX-IgM,RVIgM,CMV-IgM和HSV-Ⅱ-IgM的阳性率分别为0.00%(0/2 273),0.0.0%(0/2 273),0.88%(20/2 273)和0.00%(0/2273),TOX-IgG,RV-IgG,CMV-IgG和HSV-Ⅱ-IgG的阳性率分别为3.65%(83/2 273),86.45%(1 965/2 273),95.82%(2178/2 273)和8.27%(188/2 273),未感染TORCH病原体的新生儿占0.66%(15/2 273);TOX-IgG,RV-IgG,CM、V-IgG和HSV-Ⅱ-IgGP阳性率之间差异有统计学显著性意义(X^2=6.747,P=0.000),检出率最高的为CMV-IgG;2016年新生儿血清中TOX-IgG和CMV-IgMP阳性率均明显低于2015年(X^2=5.789~7.505,P=0.006~0.016),而2015年男性新生儿中CMV-IgM阳性率明显高于2016年(X^2=5.054,P=0.025);总体上TOX-IgG,RV-IgG,CMV-IgG和HSV-Ⅱ-IgG(TORCH-IgG)阳性率在各年度和不同性别受检者之间差异均无统计学意义(X^2=2.23~6.963,P=0.073~0.526),而2015年度女性新生儿TORCH-IgGP阳性率与2016年相比差异有统计学意义(X^2=8.247,P=0.041);2015年女性受检者TOX-IgG阳性率高于2016年女性新生儿(X^2=6.992,P=0.008);TORCH检测结果中存在单一病原体感染和多种病原体感染共六种感染模式,各年度之间以及不同性别之间TORCH感染模式差异均无统计学显著性意义(P>0.05)。结论天津地区新生儿TORCH既往感染以RV和CMV为主,新近感染皆为CMV,TORCH在不同年份及不同性别受检者中阳性率有明显变化,该研究为新生儿TORCH的流行病学以及防治提供实验资料和依据。Objective The purpose of this study was to explore the infection characteristic of Toxoplasma gondii （TOX） ,Ru- bella virus （RV）,Cytomegalovirus （CMV） and Herpes simplex virus Ⅱ type （HSV-Ⅱ ） （TORCH） infection in neonate in Tianjin area. Methods TOX-IgM/IgG, RV-IgM/IgG, CMV-IgM/IgG and HSV-Ⅱ-IgM/IgG were detected in serum of 2 273 neonate during 2015-2016 with enzyme-linked immunosorbent assay （ELISA）. Results The positive rates of TOX- IgM,RV-IgM,CMV-IgM and HSV-Ⅱ-lgM were 0.00% （0/2 273） ,0.00% （0/2 273）,0.88% （20/2 273） and 0.00% （0/2 273） ,respectively and those of TOX-IgG, RV-IgG,CMV-IgG and HSV-Ⅱ-IgG were 3.65%（83/2 273） ,86.45% （1 965/2 273） ,95.82%（2 178/2 273） and 8.27%（188/2 273） ,respectively. There was 0.66% percent （15/2 273） of examinees who were infected by none of TORCH pathogens. There existed significant statistical difference for positive rate between TOX- IgG,RV-IgG,CMV-IgG and HSV-Ⅱ-IgG （Z2= 6. 747, P = 0. 000） with consequence of the highest positive rate being CMV-IgG. The positive rates of TOX-IgG and CMV IgM in neonate of 2016 were significantly less than those in 2015 （Z2 = 5. 789-7. 505,P=0. 006-0. 016） but that of HSV-II-IgG of 2016 was statistically higher than that in 2015 （x^2 =6. 073,P =0. 014）. The positive rate of CMV-IgM in male neonate in 2015 was significantly higher than that in 2016 （x^2 =5. 054,P =0. 025）. As a whole the positive rates of TOX-IgG,RV-IgG, CMV-IgG and HSV-Ⅱ-IgG had no differences between different years,so did those between gender groups （22 = 2.23- 6. 963, P= 0. 073-0. 526）. The positive rates of TOX-IgG,RV-IgG, CMV-IgG and H SV- Ⅱ -IgG in female neonate in 2015 were statistically different from those in 2016 （x^2 = 8. 247, P = 0. 041）. The female neonate in 2015 had higher infection proportion of TOX-IgG compared with that in 2016 （x^2 = 6. 992, P=0. 008）. TORCH infection detected in 2 273 cases of neonate had one pathogen infection and multi-pathogen inf

关 键 词：新生儿 弓形体 风疹病毒 巨细胞病毒 单纯疱疹病毒II型 感染特点 

分 类 号：R722.13[医药卫生—儿科] R373[医药卫生—临床医学]