桃红四物汤对泼尼松诱导斑马鱼骨质疏松的治疗作用  

Therapeutic effect of Taohong Siwu Decoction on prednisone induced osteoporosis on zebrafish

作　　者：杨国柱[1] 段涛[1] 卢丽[1] 陈珺[1] 陆幸妍[1] 李青南[1] YANG Guozhu DUAN Tao LU Li CHEN Jun LU Xingyan LI Qingnan(School of Life Science and Biopharmacy, Guangdong Pharmaceutical University, Guangdong Provincial Key Laboratory of Biotechnology, Guangzhou 510006, China)

机构地区：[1]广东药科大学生命科学与生物制药学院,广东省生物技术候选药物研究重点实验室,广东广州510006

出　　处：《中国骨质疏松杂志》2017年第8期1007-1010,1021,共5页Chinese Journal of Osteoporosis

基　　金：广东省科技计划资助项目(2014A030304065);广东药学院科技处-附属第一医院联合自然科学培育基金项目(GYFYLH201330)

摘　　要：目的利用泼尼松诱导的斑马鱼骨质疏松模型评价中药复方桃红四物汤的抗骨质疏松的作用。方法选择发育正常的3 dpf斑马鱼胚胎,用10μg/m L泼尼松处理建立斑马鱼骨质疏松症模型,加入不同浓度(50、150和450μg/m L)的中药复方桃红四物汤。同时设置正常对照组、模型对照组和阳性对照药依替膦酸二钠组。4 d后,用0.2%钙黄绿素进行染色,在体视荧光显微镜下观察,拍照并计算斑马鱼脊椎骨的荧光强度。结果与正常对照组相比,泼尼松处理的模型对照组的斑马鱼脊椎骨荧光强度显著减少;与模型对照组比较,依替膦酸二钠能增加斑马鱼脊椎骨矿化量并有效防止泼尼松龙诱导斑马鱼产生的骨质疏松;与模型对照组比较,450μg/m L浓度的桃红四物汤能显著提高斑马鱼脊椎骨骨骼的荧光强度,而50μg/m L和150μg/m L浓度的桃红四物汤溶液组效果均不明显。结论桃红四物汤对斑马鱼的最大耐药浓度MTC为450μg/m L,并对泼尼松诱导的斑马鱼骨质疏松模型有明显的改善治疗作用。Objective To evaluate the anti-osteoporosis effect of traditional Chinese medicine Taohong Siwu Decoction by the use of prednisone-induced zebrafish osteoporosis model. Methods We selected 3 dpf normal growth zebrafish embryos,established zebrafish osteoporosis models using prednisone treatment,which were then treated with different concentrations（ 50,150 and 450μg/m L） of lyophilized powder of traditional Chinese medicine compound Taohong Siwu Decoction. At the same time,the normal control group,the model control group and the positive control group were established. After 4 days,these zebrafish were dyed for 2 h by 0. 2 % calcein,and then the fluorescence intensity of vertebrae of zebrafish were observed and calculated using stereo fluorescence microscope. Results Compared with the normal control group,prednisone-treated model group had significantly lower vertebral fluorescent density; compared with the model group,Etidronate Disodium of 300 μg/m L can increase the amount of vertebrae mineralization and effectively prevent osteoporosis induced by prednisone; compared with the model group,450 μg/m L concentration of Taohong Siwu Decoction can significantly improve the zebrafish vertebral fluorescence intensity,while the effects of the two groups of Taohong Siwu Decoction solution at 50 μg/m L and 150 μg/m L concentration were not obvious. Conclusion The Compound Chinese medicine Taohong Siwu Decoction,with a maximum concentration of drug resistance MTC of 450 μg/m L on zebrafish,had obvious therapeutic effects on zebrafish osteoporosis model induced by prednisone.

关 键 词：斑马鱼 骨质疏松 泼尼松 桃红四物汤 依替膦酸二钠 

分 类 号：R965[医药卫生—药理学]