超声造影评估兔人工骨血管化的实验研究  

Artificial bone angiogenesis in rabbits assessed with contrast-enhanced ultrasound:an experimental study

作　　者：凌茵[1] 王莎莎[2] 陈泽鹏[3] 夏远军[3] 朱贤胜[2] 范俊儿 LING Yin WANG Shasha CHEN Zepeng XIA Yuanjun ZHU Xiansheng FANG Jun＇e(Southern Medical University, Guangzhou 510515, China Department of Ultrasound, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510100, China Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510100, China)

机构地区：[1]南方医科大学,广东广州510515 [2]广州军区广州总医院超声影像科,广东广州510100 [3]广州军区广州总医院骨科,广东广州510100

出　　处：《中国医学物理学杂志》2017年第5期445-449,共5页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(51303031);广东省自然科学基金(S2012010009743)

摘　　要：目的:探讨超声在人工骨微血管生长过程中的应用价值,为骨移植生长提供早期诊断信息。方法:15只新西兰兔分别行经典双前肢桡骨大段骨缺损模型造模,植入珊瑚羟基磷灰石人工骨(CHA),分别于第3、7、11、15、30、45天对实验动物双侧CHA行常规超声及超声造影检查,观察微血管灌注情况。应用超声造影定量分析时间-强度曲线(TIC),于CHA的4个等分点分别检测基础强度(BI)、峰值强度(PI)、增强强度(△SI)、BI/PI、达峰时间(TTP),并与病理结果进行对照。结果:术后第3天超声造影显示CHA周边稍增强,中央无增强;第7天周边明显增强,中央稍增强;第15天中央见造影剂完全充盈。相同时间比较CHA的4个测量点的△SI、BI/PI、TTP值均无统计学意义(P>0.05);比较不同时间的△SI、BI/PI、TTP值均有统计学意义(P<0.05)。时间与△SI呈显著正相关,与BI/PI、TTP呈显著负相关。结论:超声造影可早期显示CHA的微血管血流灌注情况,为骨移植生长评估提供新型有价值的诊断依据。Objective To study the diagnostic value of ultrasound in artificial bone angiogenesis and provide early diagnosis information for the growth of the transplanted bones.Methods A classic bone defect model in bilateral radius was established in 15 New Zealand rabbits implanted with coral hydroxyapatite （CHA）.The microvascular blood perfusion of CHA was observed with conventional ultrasound and contrast-enhanced ultrasound （CEUS） examination on the 3rd,7th,11th,15th,30th,45th day after the bone transplantation.Each CHA was divided into 4 equal parts.Time intensity curve （TIC） of CEUS was used to detect the basic intensity （BI）,peak intensity （PI）,increased signal intensity （△ SI）,BI/PI,and time to peak （TTP） of the 4 parts,and the measurement data were compared with pathological results.Results CEUS showed that the peripheral CHA was slightly enhanced,while the central part was not enhanced on the 3rd day after the bone transplantation,but the central part was enhanced fully on the 7th day.The /△ SI,BI/PI,TTP at the same time were compared among the 4 parts in CHA and showed no significant difference （P〉0.05）,while the /△ SI,BI/PI,TTP at the different time showed obvious difference among the 4 parts （P〈0.05）.Time was positively correlated with △ SI and negatively with BI/PI and TTP.Conclusion Microvascular blood perfusion of CHA can be displayed early with the use of CEUS,which can provide a valuable diagnostic evidence for the growth assessment of transplanted bones.

关 键 词：超声造影 珊瑚羟基磷灰石 骨移植 血管化 定量分析 

分 类 号：R329.4[医药卫生—人体解剖和组织胚胎学] R445.1[医药卫生—基础医学]