HPLC法测定复方双花片中绿原酸、咖啡酸、连翘苷、穿心莲内酯和木犀草素  

Determination of chlorogenic acid, caffeic acid, forsythin, andrographalide, and luteolin in Compound Shuanghua Tablets by HPLC

作　　者：任莹 范姣姣[2] 张鑫 REN Ying FAN Jiao-jiao ZHANG Xin(Xi＇an Institute for Food and Drug Control, Xi＇an 710054, China Department of Pharmacy, Xi＇an Children＇s Hospital, Xi＇an 710003, China)

机构地区：[1]西安市食品药品检验所,陕西西安710054 [2]西安市儿童医院药剂科,陕西西安710003

出　　处：《现代药物与临床》2017年第7期1192-1195,共4页Drugs & Clinic

摘　　要：目的建立同时测定复方双花片中绿原酸、咖啡酸、连翘苷、穿心莲内酯和木犀草素的方法。方法采用Agilent 5TC-C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相:乙腈–0.4%磷酸溶液,梯度洗脱;检测波长切换0~16 min(327 nm)、16~30 min(228 nm);体积流量:1.0 mL/min;柱温:30℃,进样量:10μL。结果绿原酸、咖啡酸、连翘苷、穿心莲内酯和木犀草素在161.6~1616.0 ng(r=1.000 0)、12.48~124.80 ng(r=0.999 9)、14.30~143.04 ng(r=0.999 8)、13.48~134.80ng(r=1.000 0)、8.72~87.20 ng(r=1.000 0)线性关系良好;平均回收率分别为101.15%、98.72%、101.42%、99.15%、98.76%,RSD值分别为1.83%、2.58%、1.69%、1.20%、1.96%。结论该方法操作简单,专属性强,为更好地评价复方双花片质量提供了参考。Objective To develop a method for determination of chlorogenic acid, caffeic acid, forsythin, andrographalide, and luteolin in Compound Shuanghua Tablets by HPLC. Methods The separation was performed on Agilent 5TC-C18 column（150 mm × 4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile -0.4% phosphoric acid with gradient elution. The detection wavelengths were set at 327 nm in 0 — 16 min and 228 nm in 16 — 30 min. The flow rate was 1.0 m L/min, temperature of column was set at 30 ℃, and volume of injection was 10 μL. Results The linear range of chlorogenic acid, caffeic acid, forsythin, andrographalide, and luteolin were 161.6 — 1616.0 ng（r=1.000 0）, 12.48 — 124.80 ng（r=0.999 9）, 14.30 — 143.04 ng（r=0.999 8）, 13.48 — 134.80 ng（r=1.000 0）, 8.72 — 87.20 ng（r=1.000 0）, respectively. The average recoveries were 101.15%, 98.72%, 101.42%, 99.15%, and 98.76% with RSD 1.83%, 2.58%, 1.69%, 1.20%, and 1.96%, respectively. Conclusion The method is simple and specific, and provides a reference for quality of Compound Shuanghua Tablets.

关 键 词：复方双花片 绿原酸 咖啡酸 连翘苷 穿心莲内酯 木犀草素 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]