Theranostic gold cluster nanoassembly for simultaneous enhanced cancer imaging and photodynamic therapy  

作　　者：Hao-Dong Cui De-Hong Hu Jing-Nan Zhang Guan-Hui Gao Cui-Fang Zheng Ping Gong Xing-Hua Xi Zong-Hai Sheng Lin-Tao Cai 

机构地区：[1]Guangdong Key Laboratory of Nanomedicine,Institute of Biomedicine and Biotechnology,Shenzhen Institute of Advanced Technology,Chinese of Academy of Sciences,Shenzhen 518055,Chino [2]Departrnent of Nano Science and Technology Institute,University of Science and Technology of Chino,Suzhou 215123,China [3]Paul C Lauterbur Research Center for Biomedical Imaging,Institute of Biomedical and Health Engineering,Shenzhen Institute of Advanced Technology,Chinese of Academy of Sciences,Shenzhen 518055,China [4]Poul-Drude-lnstitut fur Festkorperelektronik,Berlin 10117,Germany [5]Department of Ophthalmology,Shenzhen People's Hospital,2nd Clinical Medical College of Jinon Univiersity,Shenzhen 518020,China

出　　处：《Chinese Chemical Letters》2017年第7期1391-1398,共8页中国化学快报（英文版）

基　　金：supported by the Major State Basic Research Development Program of China(973 Program)(No.2015CB755500);National Natural Science Foundation of China(Nos.31571013,21375141,81501580,81401521,81301272 and 81571745);Shenzhen Science and Technology Program(Nos.KQCX20140521115045447,JCYJ20150403091443298,JCYJ20130402092657771,JCYJ20150401145529015 and JCYJ20160229200902680);Instrument Developing Project of the CAS(No.YZ201439);Key International S&T Cooperation Project(No.2015DFH50230);Guangdong Natural Science Foundation of Research Team(2016A030312006)

摘　　要：As one of near-infrared（NIR） fluorescent（FL） nanoprobes, gold nanoclusters（Au NCs） are delicated to passive-targeting tumors for NIR FL imaging, but which easily cleared by the kidneys for the small size（〈1.5 nm）. Herein, the well-defined gold clusters nanoassembly（Au CNA） was synthesized by the selfassembly of Au NCs based on protein cross-linking approach. The as-prepared Au CNA demonstrated highly effective cellular uptake and precise tumor targeting compared to that of Au NCs. Moreover, with the irradiation of 660 nm laser, Au CNA generated largely reactive oxygen species（ROS） for photodynamic therapy（PDT）. In vitro and [39TD$IF]in vivo PDT revealed that Au CNA exhibited largely cell death and significantly tumor removal at a low power density of 0.2 W/cm^2. It could be speculated that the laser-excited Au CNA produced photon energy, which further obtained electron from oxygen to generate radical species.Therefore, Au CNA as a photosensitizer could realize NIR FL imaging and NIR laser induced PDT.As one of near-infrared（NIR） fluorescent（FL） nanoprobes, gold nanoclusters（Au NCs） are delicated to passive-targeting tumors for NIR FL imaging, but which easily cleared by the kidneys for the small size（〈1.5 nm）. Herein, the well-defined gold clusters nanoassembly（Au CNA） was synthesized by the selfassembly of Au NCs based on protein cross-linking approach. The as-prepared Au CNA demonstrated highly effective cellular uptake and precise tumor targeting compared to that of Au NCs. Moreover, with the irradiation of 660 nm laser, Au CNA generated largely reactive oxygen species（ROS） for photodynamic therapy（PDT）. In vitro and [39TD$IF]in vivo PDT revealed that Au CNA exhibited largely cell death and significantly tumor removal at a low power density of 0.2 W/cm^2. It could be speculated that the laser-excited Au CNA produced photon energy, which further obtained electron from oxygen to generate radical species.Therefore, Au CNA as a photosensitizer could realize NIR FL imaging and NIR laser induced PDT.

关 键 词：Gold nanoclusters Self-assembly Near-infrared fluorescent nanoprobe In vivo imaging Photodynamic therapy 

分 类 号：TQ460.1[化学工程—制药化工]