Energy-coupling mechanism of the multidrug resistance transporter AcrB： Evidence for membrane potential-driving hypothesis through mutagenic analysis  

作　　者：Liu, Min Zhang, Xuejun C. 

机构地区：[1]Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China [2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

出　　处：《Protein & Cell》2017年第8期623-627,共5页蛋白质与细胞（英文版）

基　　金：The authors thank Dr. Yong Tao of the Institute of Microbiology, CAS, for providing the ΔacrB cell strain. We thank Dr. Torsten Juelich for linguistic assistance during the preparation of this manuscript. This work was supported by the National Basic Research Program （973 Program） （No. 2015CB910104 to XCZ） and National Natural Science Foundation of China （Grant No. 31470745 to XCZ）.

摘　　要：Dear Editor, RND （resistance nodulation-cell division） exporters are widely expressed across the lineage of Gram-negative bacteria （Fig. S1 ）. Their functional role is to utilize the protonmotive force of the cell to drive the expulsion of cytotoxic substances from the outer leaflet of the inner membrane as well as the periplasm to extracellular space （Yamaguchi et al., 2015）.

关 键 词：突变分析 耦合机制 ACRB 驱动 膜电位 转运体 证据 假说 

分 类 号：Q244[生物学—细胞生物学] TP311.13[自动化与计算机技术—计算机软件与理论]