正交试验优化新疆芫荽中总黄酮提取工艺  

Optimization of Technological Parameters for Extraction of Total Flavonoids from Coriandrum sativum L. by Orthogonal Experiment

作　　者：阿依古丽.塔什波拉提 故丽巴哈.艾合买提 阿不力孜.艾力 AYGUL Tashpolat GULIBAHA Aihemaiti ABLIZ Aili(Xinjiang Uygnr Autonomous Region Academy of Instrumental Analysis, Urumqi, Xinjiang 830011)

机构地区：[1]新疆维吾尔自治区分析测试研究院,新疆乌鲁木齐830011

出　　处：《安徽农业科学》2017年第22期1-4,16,共5页Journal of Anhui Agricultural Sciences

基　　金：新疆乌鲁木齐市科技局种子基金项目(k111410002)

摘　　要：[目的]优化新疆芫荽中总黄酮提取工艺。[方法]以新疆芫荽地上部分为原料,采用单因素试验和正交试验设计,以紫外分光光度法测定提取液中总黄酮提取率。[结果]新疆芫荽地上部分总黄酮优化后提取工艺:提取溶剂(乙醇)浓度为40%,提取时间为120 min,固液比为1∶15,提取温度为60℃,粒度为40目,提取次数为2。在上述工艺条件下,黄酮提取率达3.28%。[结论]采用正交试验设计优化后的新疆芫荽地上部分总黄酮的提取工艺是合理可行的。[ Objective ] The aim was to optimize technological parameters for extraction of total flavonoids from Coriandrum sativum L.. [ Method ] Aerial parts of Coriandrum sativum L. as raw material, using single factor experiment and orthogonal experiment design, the extraction ratio of total flavonoids from the Coriandrum sativum L. was deter mined by UV-Vis spectrophotometry, and the technology parameters of extraction of total flavonoids from the Coriandrum sativum L. were studied. [ Result] The optimal extraction process conditions were as followed： 40% of solvent concentration, 120 rain of extraction time, 1 ： 20 of solid-liquid ratio, 60℃ of extraction temperature, 40 mesh of material size and twice extraction, above the optimal conditions, the extraction ratio of total flavonoids from the Coriandrum sativum L. was 3.28%. [ Conclusion] The optimized technological parameters for extraction of total flavonoids from Coriandrum sativum L. by orthogonal experiment is reasonable and feasible.

关 键 词：新疆芫荽 醇提取工艺 总黄酮 紫外可见光谱仪 

分 类 号：S573.2[农业科学—作物学]