中草药白头翁中总黄酮提取工艺研究  

Study on Technology of Extracting Total Flavonoids from Radix Pulsatillae

作　　者：雒江菡[1] 于瑞洪[2] LUO Jiang-han YU Rui-hong(Institute of Cell and Moleeular Biology, Harbin University of Commerce, Harbin, Heilongjiang 150076 Heilongjiang Vocational College of Biology Science and Technology, Harbin, Heilongjiang 150025)

机构地区：[1]哈尔滨商业大学,细胞与分子生物学研究所,黑龙江哈尔滨150076 [2]黑龙江生物科技职业学院,黑龙江哈尔滨150025

出　　处：《安徽农业科学》2017年第22期88-89,170,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究中草药白头翁中总黄酮的提取工艺。[方法]以白头翁为原料提取总黄酮,通过对影响白头翁中总黄酮得率的4种因素(料液比、提取温度、提取时间和乙醇浓度)进行单因素试验,确定白头翁中提取总黄酮的最佳工艺。[结果]白头翁中提取总黄酮的最佳条件为:料液比为1∶20,提取温度为70℃,提取时间为3 h,乙醇浓度为70%,此时,总黄酮得率为8.29%。[结论]该研究为建立白头翁黄酮的质量标准、有效地控制白头翁药材的质量奠定基础。[ Objective ] The research aimed to study the technology of extracting total flavonoids from Radix Pulsatillae. [ Method ] Using Radix Pulsatillae as raw material to extract total flavonoids, the optimum extraction technology of total flavonoids from Radix Pulsatillae was determined by single factor test of four factors （solid-liquid ratio, extraction temperature, extraction time and ethanol concentration） affecting the total flavonoid yield. [ Result] The total flavonoid yield was 8.29% when the optimum extraction conditions were as follows：1： 20 solid-liquid ratio, extraction temperature of 70℃, extraction time of 3 h, ethanol concentration of 70%. [ Conclusion] This study laid the foundation for the establishment of quality standards for Radix Pulsatillae flavonoids and effective control of the quality of Radix PulsatiUae.

关 键 词：白头翁 总黄酮 单因素试验 提取工艺 

分 类 号：S567.23[农业科学—中草药栽培]