虎皮鹦鹉全基因组中微卫星分布规律研究  

Distribution of Microsatellites in Genome of Budgerigar( Melopsittacus undulatus)

作　　者：黄杰[1] 原宝东[1] 杨承忠[2] 

机构地区：[1]商丘师范学院生物与食品学院,商丘476000 [2]重庆师范大学生命科学学院,重庆401331

出　　处：《野生动物学报》2017年第3期422-426,共5页CHINESE JOURNAL OF WILDLIFE

基　　金：国家自然科学基金项目(No.31501845);商丘师范学院高层次人才科研启动项目(No.50013901;50014001)

摘　　要：对虎皮鹦鹉(Melopsittacus undulatus)全基因组中微卫星分布特征进行了分析,查找到l^6个碱基重复类型的微卫星序列共90 346个,约占整个基因组总长度的序列(1.1Gb)的0.41%,分布频率为82.9/Mb。不同类型微卫星中,单碱基重复类型数目最多,为50 349个,占总数的55.7%;其次是二、四、三、五、六碱基重复单元序列,分别占到总数的16.3%,13.7%,10.8%,2.9%,0.5%。单碱基微卫星中A重复类型数量最多,二碱基中AT最多,三碱基中AAT,四碱基中AAAC最多,五碱基中AAAGA最多,六碱基中AACCCT最多。A、AT、C、AC、AAT、AAAC、AAAT、AGG、AGC、AG、AAAG、AAC、ATCC、AAGG、AGAT、ATC、AACC、ACC依次是虎皮鹦鹉基因组中分布数量最多的18种微卫星重复拷贝类型。本研究将为虎皮鹦鹉微卫星标记的分离筛选、遗传多样性的研究以及不同物种微卫星的比较分析奠定了基础。We analyzed the distribution of microsatellites in the entire genome of budgerigar（ Melop- sittacus undulatus）. A total of 90 346 simple sequence repeats（SSRs） with 1 -6 bp nucleotide motifs were identified, means that about 0.41% of the budgerigar genome was occupied by SSRs. And the frequency of SSR was 82.9/Mb. In the different repeat types of the microsatellites, mononucleotide repeat motifs was the most abundant type（55.7% ）, while the tetranucleotide, dinucleotide, trinucleotide, pentanucleotide and hexanucleotide repeats were 16.3%, 13.7%, 10.8%, 2.9% and 0.5%, respectively. Moreover, A, AT, AAT, AAAC, AAAGA, AACCCT were the most frequent repeat copy units of the six motif types, respectively. In the budgerigar genome, A, AT, C, AC, AAT, AAAC, AAAT, AGG, AGC, AG, AAAG, AAC, ATCC, AAGG, AGAT, ATC, AACC, and ACC were the predominant repeat types. This study will facilitate the isolation of microsatellites and the genetic diversity analysis of budgerigar, and serve as a foundation for comparative studies of microsatellites in different species.

关 键 词：虎皮鹦鹉 基因组 微卫星 

分 类 号：Q78[生物学—分子生物学] Q953