抵当汤对糖尿病兔腹主动脉球囊损伤后血管重构的研究  

Didang Tang Can Improve Vascular Remodeling of Diabetic Rabbits with Abdominal Aortic Balloon Injury

作　　者：曹明满[1] 吕雄[2] 陈婧[1] 郑艳华[1] 庞如菲 高洁嫦[1] 肖姬[1] 

机构地区：[1]广州医科大学附属第二医院(番禺院区中医科),广东广州511447 [2]广东省第二中医院,广东广州510095

出　　处：《新中医》2017年第8期11-15,共5页New Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81403244)

摘　　要：目的 :探讨抵当汤对糖尿病兔腹主动脉球囊损伤后血管重构的作用及机制。方法 :首先建立糖尿病兔模型,分为假手术组、模型组、抵当汤低剂量组、抵当汤高剂量组、Y-27632组,除假手术组外,其他各组兔接受球囊损伤手术,建立糖尿病实验兔腹主动脉球囊损伤模型。模型组接受生理盐水胃管注入;Y-27632组接受Y-27632胃管注入,给药剂量为70 mg/(kg·d);抵当汤低剂量组、抵当汤高剂量组分别给予8.1 g/(kg·d)、16.2 g/(kg·d)的抵当汤胃管注射。术后通过血管影像学、病理学方法观察抵当汤对糖尿病实验兔腹主动脉球囊损伤后血管内径、内膜、中膜、血流速度等的影响。结果:与假手术组比较,模型组动脉内径和血流速度均明显降低,管腔面积和内膜包绕面积显著减少,内膜面积和中膜面积显著增加,差异均有统计学意义(P<0.05,P<0.01),说明以动脉内膜增殖及管腔狭窄为特征的糖尿病实验兔腹主动脉球囊损伤后动脉损伤模型造模成功。与模型组比较,Y-27632组的动脉内径显著升高,抵当汤高剂量组和抵当汤低剂量组的动脉内径和血流速度均显著升高,Y-27632组、抵当汤低剂量组、抵当汤高剂量组的中膜面积显著降低,抵当汤低剂量组和抵当汤高剂量组的内膜包绕面积均增加,差异均有统计学意义(P<0.05,P<0.01)。与Y-27632组比较,抵当汤高剂量组血流速度显著升高,内膜面积和中膜面积显著降低,内膜包绕面积显著增加,差异均有统计学意义(P<0.05)。与抵当汤低剂量组比较,抵当汤高剂量组血流速度显著升高,内膜面积和中膜面积显著降低,差异均有统计学意义(P<0.05,P<0.01)。结论:抵当汤可以改善糖尿病实验兔腹主动脉球囊损伤后的血管重构。Objective. To explore the function and the mechanism of Didang tang on vascular remodeling of diabetic rabbits with abdominal aortic balloon injury. Methods. Adopted diabetic rabbits firstly, and divided the model rabbits into sham-operated group, model group, low and high dose of resistance decoction group, Y-27632 group. Except the sham-operated group, others were given balloon injury surgery to establish abdominal aortic balloon injury models of diabetic rabbits. The model group was given normal saline by gastric tube influx; Y-27632 group was given Y-27632 with the dosage of 70mg/（kg.d） by gastric tube influx; the low and high dose groups of Didang tang were given Didang tang with the dosage of 8.1mg/（kg·d） and 16.2 mg/ （kg·d） respectively by gastric tube injection. After surgery, observed the effect of Didang tang on blood vessel diameter, endangium, tunica media, and blood flow velocity of abdominal aortic balloon injury models of diabetic rabbits by angiography and pathological method. Results. Comparing with sham-operated group, the arterial diameter and blood flow velocity of model group were decreased significantly lumen areas and internal elastic area were reduced significantly, while the areas of intima and tunica media were increased significantly, the differences all being significant （P 〈0.05, P 〈0.01）. Both of the features of intimal proliferation and luminal stenosis indicated that the abdominal aortic balloon injury models of diabetic rabbits were successful. Comparing with model group, arterial diameter of Y-27632 group were increased significantly~ both arterial diameter and blood flow velocity of high dose group of Didang tang and low dose group of Didang tang were increased significantly; tunica media of Y-27632 group, low and high dose groups of Didang tang were decreased significantly internal elastic area of both low and high dose groups of Didang tang was increased significantly, the differences all being significant （P〈0.05, P〈0.01）. Comparing with the Y-27

关 键 词：糖尿病血管病变 抵当汤 中药疗法 血管重构 疾病模型 新西兰大耳白兔 

分 类 号：R587.2[医药卫生—内分泌] R285.5[医药卫生—内科学]