丹芪通脉破壁饮片颗粒对apoE-/-小鼠主动脉粥样硬化影响的实验研究  

Danqi Tongmai Wall-broken Decoction Pieces Granules Have Improving Effect on Aortic Atherosclerosis of Apo E-/-Mice

作　　者：李亮[1] 张俊杰[1] 张文霞[1] 王家赐[1] 林红军[1] 陈巧玲[1] 朱盈盈[1] 

机构地区：[1]广州中医药大学附属中山医院,广东中山528400

出　　处：《新中医》2017年第8期19-22,共4页New Chinese Medicine

基　　金：中山市科研计划项目(2014A1FC170);广东省中医药局项目(20141283)

摘　　要：目的 :观察丹芪通脉破壁饮片颗粒对apo E-/-小鼠血脂及氧化应激指标的影响,初步探讨其对主动脉粥样硬化的作用机制。方法:将40只apo E-/-小鼠以高脂饲料喂养8周构建动脉粥样硬化小鼠模型,随机分为模型组、饮片组、低浓度颗粒组、中浓度颗粒组、高浓度颗粒组,每组8只,另取8只C57BL/6雄性小鼠为空白对照组。饮片组以丹芪饮片煎剂12 g/(kg·d)灌胃,低浓度颗粒组、中浓度颗粒组及高浓度颗粒组分别以1.5、3、6 g/(kg·d)浓度灌胃丹芪通脉破壁饮片颗粒,模型组和空白对照组灌胃等量生理盐水。空白对照组以普通饲料喂养,其余各组小鼠均以高脂高胆固醇饲料喂养。ELISA法检测血清血脂四项,检测主动脉组织超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)含量,HE染色观察小鼠胸主动脉管腔内粥样斑块。结果:与空白对照组比较,模型组小鼠从第4周开始体质量显著升高,血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)水平均显著升高,小鼠主动脉组织SOD和NO浓度显著下降,MDA浓度显著升高;各给药组小鼠TC、TG、LDL-C及HDL-C水平均显著升高,差异均有统计学意义(P<0.05,P<0.01)。与模型组比较,从第4周开始,饮片组及各浓度颗粒组小鼠体质量显著减少,饮片组、中浓度颗粒组、高浓度颗粒组TC、TG、LDL-C、MDA水平均显著下降,主动脉组织SOD和NO浓度均显著升高,低浓度颗粒组SOD和NO浓度均显著升高,差异均有统计学意义(P<0.05,P<0.01)。结论:丹芪饮片煎剂及丹芪通脉破壁饮片颗粒均具有改善血脂谱、减轻氧化应激及抗动脉粥样硬化等作用,疗效一致。objective： To observe effect of Danqi Tongmai wall-broken pieces granules on serum lipids and oxidative stress indexes of apoE-/-mice, and to do preliminary discussion of its mechanism on aortic atheroscterosis. Methods： Selected 40 apoE-/-mice and fed them with high fat diet for eight weeks to establish mice model of artherosclerosis, and divided them into the model group, pieces group, and low, medium and high concentration granule groups, being 8 cases in each. Another 8 C57BL/6 male mice were selected as the blank control group. The pieces group was given Danqi decoction pieces of 12 g/（kg·d） by gavage, low concentration granule group, medium concentration granule group and high concentration granule group were given Danqi Tongmai wall-broken pieces granules of 1.5 g/（kg·d）, 3 g/（kg·d） and 6 g/（kg·d） respectively by gavage; the model group and the blank control group were given equivalent saline solution. The blank control group was given normal diet while the other groups were given high-fat and cholesterolrich diet. Detected serum and blood lipid indexes with enzyme linked immunosorbent assay （ELISA）, and detected content of superoxide dismutase （SOD）, malondialdehyde （MDA） and nitric oxide （NO） in aortic tissue, and observed atherosclerotic plaque of the mice with HE staining. Results： Compared with the blank control group, from the fourth week, body weight, levels of Triglyceride （TG）, total cholesterol （TC）, high density lipoprotein （HDL-C） and low density lipoprotein （LDL-C） of the mice in model group were increased significantly., concentration of SOD and NO in aortic tissue of the mice in model group was significantly reduced, while concentration of MDA was increased significantly; levels of TC, TG, LDL-C and HDL-C in mice of the other groups were increased significantly（P 〈 0.05, P〈 0.01）. Compared with the model group, from the fourth week, body weight of the mice in the low, medium and high concentration granule groups and the pieces

关 键 词：丹芪通脉破壁饮片颗粒 动脉粥样硬化 血脂 氧化应激 动物实验 APOE-/-小鼠 C57BL/6小鼠 

分 类 号：R285.5[医药卫生—中药学]