辅酶Q10干预糖尿病牙周炎大鼠血清骨指标的变化  

Effect of coenzyme Q10 on expression level of OPG/RANKL in serum and periodontal tissue in type2 diabetic rats with experimental periodontitis

作　　者：徐艳丽[1] 吴仲寅[1] 薛毅[1] XU Yan-li WU Zhong-yin XUE Yi.(Department of Stomotalogy, People＇ s Liberation Army, Bethune International Peace Hospital, Hebei 050082, Chin)

机构地区：[1]解放军白求恩国际和平医院,医师河北050082

出　　处：《口腔颌面修复学杂志》2017年第4期202-206,共5页Chinese Journal of Prosthodontics

基　　金：全军"十二五"面上课题(项目编号:CWS11J219)

摘　　要：目的:探索辅酶Q10对伴2型糖尿病牙周炎大鼠血清RANKL、OPG的水平,及牙周病损中的作用。方法:选择质量200g左右Wistar健康雄性清洁级大鼠48只,随机分为3组:糖尿病对照组(DG),牙周炎+糖尿病+NaCl组(PDNaG),牙周炎+糖尿病+Co Q10组(PDCoG)。观察大鼠的牙周探诊深度,牙槽骨垂直吸收高度,H E染色检测牙周组织病理变化。酶联免疫吸附法(ELISA)检测血清R ANKL/OPG水平。结果:灌胃第8周,各组大鼠的牙周探诊深度、牙槽骨垂直吸收高度差异均有统计学意义(P<0.05);各组血清RANKL、OPG水平差异无统计学意义(P>0.05);灌胃第8周,各组间RANKL/OPG的比值差异有统计学意义(P<0.05)。结论:辅酶Q10对伴2型糖尿病牙周炎大鼠牙周炎有改善作用,可调节血清R ANKL/OPG达到平衡。Objective： To investigate the effect of CoQ10 to the periodontal tissue with diabetes periodontitis by monitoring the damage of periodontitis and measuring the level of RANKL and OPG in serum. Methods： 48 healthy and SPF Wistar rats were randomly divided into 3 groups, diabetes mellitus group （DG）, periodontitis and diabetes mellitus with physiological saline group （PDNaG） and periodontitis and diabetes mellitus with CoQ10 group （PDCoG）. PDNaG and PD- CoG set up for periodontitis model with silk ligatured around the dental cervix, and DG, PDNaG and PDCoG established the diabetes mellitus model with fed high-fat and high-sugar diet＋injection of streptozocin （STZ）. The mean depth of periodontal pocket and total attachment loss of rats each group were measured and analyzed statistically before administered and 2th, 4th, 8th week after administering. The gingival tissue was observed with microscope after HE stained, and Enzyme-Linked Immuno Sorbent Assay（ELISA） for RANKL/OPG levels. Results： The index of mean depth of periodontal pocket and total attachment loss showed significantly different during DG, PDNaG and PDCoG at the 8th week （P〈0.05）, the level of RANKL and OPG in serum showed no significantly different during DG, PDNaG and PDCoG at the 8th week （P〉0.05）, but the ratio of RANKL/OPG had significant difference during three groups at the 8th week （P〈0.05）. Conclusion： The experiment results indicated that administration with a certain dose of CoQ 10 could improve periodontal local indexes of rats, slowing attachment loss and alveolar bone resorption,and could make the ratio of RANKL/OPG in serum.

关 键 词：牙周炎 2型糖尿病 辅酶Q10 核因子-KB受体活化子配体 骨保护因子 

分 类 号：R587.2[医药卫生—内分泌] R781.42[医药卫生—内科学]