TLR4和CXCR7在胃癌组织中的表达及与幽门螺杆菌感染的关系  

Expression of TLR4 and CXCR7 in gastric carcinoma and its relationship with HP positive infection

作　　者：蔡兆根[1] 承泽农[1] 李大鹏[2] 

机构地区：[1]蚌埠医学院第一附属医院病理科蚌埠医学院病理学教研室安徽省感染与免疫重点实验室,安徽省蚌埠233003 [2]蚌埠医学院第一附属医院病理科蚌埠医学院消化内科,安徽省蚌埠233003

出　　处：《中国基层医药》2017年第16期2422-2426,J0003,共6页Chinese Journal of Primary Medicine and Pharmacy

基　　金：安徽省高校自然科学研究重点项目（KJ2017A236）

摘　　要：目的探讨Toll样受体4（TLR4）和趋化因子受体7（CXCR7）在幽门螺杆菌（HP）阳性胃癌发生、发展过程中的作用及机制.方法应用免疫组化SP法检测147例胃癌组织及60例癌旁正常组织标本中TLR4和CXCR7蛋白表达情况;同时应用免疫组化和革兰氏染色法检测上述组织中HP感染情况;分析TLR4和CXCR7蛋白表达与胃癌主要临床病理指标的关系.结果胃癌组织中TLR4和CXCR7蛋白阳性率分别为63.9%和62.6%,均显著高于正常对照组的15.0%和5.0%（X2=40.832、56.901,均P〈0.01）;有淋巴结转移者TLR4、CXCR7阳性率显著高于无淋巴结转移者（X2=9.809、11.444,均P〈0.01）;TNM分期为Ⅲ＋Ⅳ期者TLR4、CXCR7阳性表达率明显高于Ⅰ＋Ⅱ期者（X2=24.927、27.642,均P〈0.01）;同时,两者在HP阳性胃癌组均高于HP阴性组.Kaplan-Meier生存分析显示,TLR4和CXCR7过表达的患者生存率明显低于TLR4和CXCR7表达阴性者（F=4.053、4.091,均P〈0.01）.COX回归分析显示,TNM分期、TLR4和CXCR7的过表达是影响胃癌根治术后患者预后的独立因素.结论TLR4和CXCR7与胃癌的发生发展密切相关,在HP致癌中起到重要的作用;可能通过LPS/TLR4信号通路参与胃癌细胞的侵袭和转移.Objective To explore the role and mechanism of Toll-like receptor 4（TLR4）and chemokine receptor 7（CXCR7）in the pathogenesis and development of Helicobacter pylori（HP）positive gastric cancer.Methods Tissues of 147 cases with gastric carcinoma and 60 normal control were collected.The protein expression of TLR4,CXCR7 was detected by immunohistochemical staining.HP infection in these samples were detected by immunohistochemistry and Gram staining.Results The positive rates of TLR4 and CXCR7 in gastric cancer tissues were 63.9% and 62.6%,respectively,which were significantly higher than those in normal control group（15.0% and 5.0%,respectively;X2=40.832,56.901,all P〈0.01）.The positive rates of TLR4 and CXCR7 in patients with lymph node metastasis were significantly higher than those without lymph node metastasis（X2=9.809,11.444,all P〈0.01）.The positive rates of TLR4 and CXCR7 in patients with stage Ⅲ and Ⅳ were significantly higher than those of stageⅠand Ⅱ（X2=24.927,27.642,all P〈0.01）.The expression of TLR4 and CXCR7 in gastric carcinoma was significantly related to HP infection.Kaplan-Meier survival analysis showed that the survival rates in TLR4 and CXCR7 positive group were significantly lower than those in TLR4 and CXCR7 negative groups（F=4.053,4.091,all P〈0.05）.COX regression analysis indicated that the TNM stage,the expression of TLR4 and CXCR7 were independent prognosis factors of gastric carcinoma.Conclusion TLR4 and CXCR7 are closely related to the occurrence and development of gastric cancer,and may play an important role in HP carcinogenesis.It may be involved in the invasion and metastasis of gastric cancer cells via LPS/TLR4 signaling pathway.

关 键 词：胃肿瘤 螺杆菌 幽门 TOLL样受体4 受体 趋化因子 

分 类 号：R516[医药卫生—内科学] R735.2[医药卫生—临床医学]