血浆同型半胱氨酸及亚甲基四氢叶酸还原酶基因多态性与广西玉林地区汉族原发性高血压患者的关系  

Relation of plasm homocysteine and methylenetetrahydrofolate reductase gene polymorphisms in patients with essential hypertension in Han in Yulin of Guangxi

作　　者：赵艳梅[1] 李平[1] 许为炎[1] 谢文超[1] 林智海[1] 黄翠娟[1] 梁萍[2] 刘康海[3] 

机构地区：[1]玉林市第一人民医院心内科,广西壮族自治区玉林市537000 [2]玉林市第一人民医院体检中心,广西壮族自治区玉林市537000 [3]玉林市第一人民医院检验科,广西壮族自治区玉林市537000

出　　处：《中国心血管病研究》2017年第6期528-532,共5页Chinese Journal of Cardiovascular Research

摘　　要：目的 探讨广西玉林地区汉族原发性高血压（EH）患者血浆同型半胱氨酸（Hcy）水平及其代谢酶N5,10-亚甲基四氢叶酸还原酶（MTHFR）C677T基因多态性的临床意义.方法 选择玉林汉族原发性高血压患者200例作为EH组,健康体检血压正常者200例作为对照组（NT组）.所有受试者抽取外周静脉血用全自动生化分析仪检测Hcy及其他生化指标的水平;同时提取DNA,采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的方法检测两组MTHFR C677T基因型.结果 两组Hcy水平比较差异有统计学意义（P〈0.05）.CC、CT、TT三种基因型频率表达在EH组和NT组中分别为27％、49.5％、23.5％和40％、45.5％、14.5％,CT突变频率高;EH组中C和T等位基因为51.75％和48.25％,NT组中为62.75％和37.25％.两组三种基因型总体频率及等位基因频率分布差异有统计学意义（P〈0.05）,两组TT型及T等位基因频率比较差异有统计学意义（P〈0.05）.EH组中TT型Hcy水平高于CC型和CT型.Logistic回归显示,CT＋TT基因型、Hcy可能为EH的危险因素.结论 Hcy水平升高及MTHFR C677T基因多态性可能与广西玉林地区汉族EH的发生有关.T等位基因可能为该人群易感基因,二者可能对该地区EH的预测、诊疗有一定临床价值.Objective Discussion the relationship of plasma homocysteine （Hcy） levels and N 5,10-methylenetetrahydrofolate reductase （MTHFR） C677T polymorphism and essential hypertension in Han in Yulin of Guangxi.Methods A total of 400 Han nationality of Yulin,peripheral blood in 200 EH patients and 200 normaltensive （NT） subjects.The homocysteine and other biochemical indicators levels were detected of automatic biochemical analyzer.Extracting DNA,the MTHFR C677T genotype in two groups were discover with polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.Results Hcy levels of the two groups was statistically significant （P〈0.05）.The frequency expressed of CC,CT,TT three genotypes in EH group and NT group,respectively 27%,49.5%,23.5% and 40%,45.5%,14.5%,CT high mutation frequency.The frequency of C and T allele was 51.75% and 48.25% in EH group,and 62.75% and 37.25% in NT group.Three genotype frequencies and allele genes of the two groups was statistically significant（P〈0.05）.The frequency of genotype TT and T allele of two groups was statistically significant（P〈0.05）.In EH group,the level of Hcy type TT was higher than that of type CC and type CT.Logistic regression showed that CT＋TT genotype,Hcy may be.a risk factor for EH.Conclusion Elevated levels of Hcy and the MTHFR C677T gene polymorphism may be related to the occurrence of EH in the Han nationality in Yulin area of Guangxi,the T allele may be a susceptibility gene in this population.Hcy level,MTHFR C677T gene polymorphism may have a certain clinical value for the prediction and treatment of EH in the region.

关 键 词：同型半胱氨酸 亚甲基四氢叶酸还原酶 基因多态性 高血压 广西玉林地区汉族 

分 类 号：R544.1[医药卫生—心血管疾病]