中胚层同源盒基因1在急性心肌梗死大鼠心脏微血管内皮的表达变化  

Expression of mesodermal homobox gene 1 in cardiac microvascular endothelial cells in rats with acute myocardial infarction

作　　者：沈俊[1,2] 袁平[1] 唐俊明[2] 张蕾[2] 赵继先[1] 张焕鑫[1] 薛仕珍[1] 冯怡[1] 王家宁[1,2] 

机构地区：[1]湖北医药学院附属人民医院心内科1病区,湖北省十堰市442000 [2]湖北医药学院附属人民医院临床医学研究所,湖北省十堰市442000

出　　处：《中国心血管病研究》2017年第8期757-759,I0002,共4页Chinese Journal of Cardiovascular Research

基　　金：国家自然科学基金（项目编号：81270221）

摘　　要：目的 研究中胚层同源盒基因1（Meox1）在急性心肌梗死大鼠心脏微血管内皮的表达情况.方法 成年雄性SD大鼠20只随机分成急性心肌梗死组和假手术组,各10只.采用结扎左冠状动脉前降支结扎法构建急性心肌梗死模型,假手术组仅在左冠状动脉前降支穿线不结扎.建模后将急性心肌梗死组作为实验组,假手术组作为对照组.建模后第7天处死两组大鼠,进行免疫组化染色,分析急性心肌梗死后心脏微血管上Meox1的表达情况.结果 通过免疫组化分析发现,在心肌梗死区及梗死边缘区微血管内皮可见棕黄色颗粒沉积,提示有Meox1表达;在假手术组及梗死远离区微血管内皮上未见Meox1的表达.结论 在急性心肌梗死大鼠心脏微血管内皮上Meox1表达增多,推测其可能参与心肌梗死后的血管重塑.Objective To study the expression of mesoderm homebox gene 1 （Meox1） in the microvascular endothelium of acute myocardial infarction rats.Methods Twenty adult male SD rats were randomly divided into acute myocardial infarction group and sham operation group.The model of acute myocardial infarction was established by ligating the left anterior descending coronary artery.The sham operation group was not ligated only in the anterior descending branch of the left coronary artery.The acute myocardial infarction group was used as the experimental group and the sham operation group as the control group.Two groups of rats were sacrificed on the 7th day after modeling.Immunohistochemical staining was performed to analyze the expression of Meox1 on the cardiac microvessels after acute myocardial infarction.Results The expression of Meox1 was observed by immunohistochemical analysis in the microvascular endothelium in the infarcted and infarcted areas,suggesting that Meox1 expression was not found in the sham operation group and the infarcted lesion.Conclusion The expression of Meox1 in the heart of patients with acute myocardial infarction increased,suggesting that it may be involved in vascular remodeling after myocardial infarction.

关 键 词：心肌梗死 同源盒基因Meox1 表达 

分 类 号：Q95-33[生物学—动物学] R542.2[医药卫生—心血管疾病]