亚硝酰氢对冠心病心力衰竭大鼠心肌线粒体功能的影响  

Effect of nitroxyl hydrogen on myocardial mitochondrial function in rats with heart failure of coronary heart disease

作　　者：郭彦青 徐继尧 王日军 王敬萍 

机构地区：[1]山西省心血管病医院心内科,山西省太原市030024

出　　处：《中国心血管病研究》2017年第8期760-763,共4页Chinese Journal of Cardiovascular Research

基　　金：山西省应用基础研究项目（面上青年基金项目）（项目编号：201601D021156）

摘　　要：目的 探讨亚硝酰氢对心肌梗死后心力衰竭大鼠心肌线粒体Na＋,K＋-ATP酶、Ca2＋-ATP酶及线粒体呼吸链复合体Ⅰ、Ⅳ活性的影响.方法 结扎大鼠前降支制作心肌梗死后心力衰竭模型,35只Wistar大鼠随机分为假手术组、心力衰竭模型组、亚硝酰氢组.分别给予各组大鼠皮下注射0.9％生理盐水2ml或亚硝酰氢供体0.1 mg/100 g,4周后差速离心法提取线粒体,测量其Na＋、K＋-ATP酶及Ca2＋-ATP酶活性;应用紫外分光光度法测定缺血再灌注后心肌线粒体呼吸链复合体Ⅰ、Ⅳ的活性.结果 与模型组相比,亚硝酰氢组大鼠线粒体Na＋,K＋-ATP酶[（42.64±4.13）U/mgprot比（37.99±3.25） U/mgprot]、Ca2＋-ATP酶[（32.44±3.13） U/mgprot 比（27.91 ±4.25）U/mgprot]活性及线粒体呼吸链复合体Ⅰ、Ⅳ活性[（0.554±0.052）ukat/g比（0.413±0.060）ukat/g、（0.058±0.005）ukat/g比（0.051±0.004）ukat/g]明显增加.结论 亚硝酰氢改善心力衰竭大鼠心功能的作用通过增加线粒体Na＋,K＋-ATP酶和Ca2＋-ATP酶活性,以及线粒体呼吸链复合体Ⅰ、Ⅳ活性而实现.Objective To investigate the effects of nitroxyl hydrogen on activity of Na＋,K＋-ATP enzyme and Ca2＋-ATP enzyme and mitochondrial respiratory chain complex Ⅰ and Ⅳ in rats with heart failure after myocardial infarction.Methods Rats heart failure model was made by ligated left coronary artery anterior descending branch.35 Wistar rats were randomly divided into 3 groups including sham-operated group,heart failure group,treated with hydrogen nitrosyl group.The rats were given subcutaneous injection of 0.9％ 2 ml saline or nitrosyl hydrogen donor 0.1 mg/100 g for 4 weeks.The activity of Na＋,K＋-ATP enzyme and Ca2＋-ATP enzyme and mitochondrial respiratory chain complex Ⅰ and Ⅳ was measured by using ultraviolet spectrophotometry after centrifugation of mitochondria.Results The activity of Na＋,K＋-ATP enzyme [（37.99±3.25）U/mgprot vs （42.64±4.13）U/mgprot] and Ca2＋-ATP enzyme [（27.91±4.25）U/mgprot vs （32.44±3.13）U/mgprot] and mitochondrial respiratory chain complex Ⅰ and Ⅳ [（0.413±0.060）ukat/g vs （0.554±0.052）ukat/g,（0.051±0.004）ukat/g vs （0.058±0.005）ukat/g] in HNO group was increased significantly compared with the model group.Conclusion HNO improves cardiac function of heart failure rats by increasing mitochondrial activity of Na＋,K＋-ATP enzyme and Ca2＋-ATP enzyme and mitochondrial respiratory chain complex Ⅰ and Ⅳ.

关 键 词：亚硝酰氢 Na+ K+-ATP酶 CA2+-ATP酶 呼吸链 

分 类 号：Q95-33[生物学—动物学] R541.4[医药卫生—心血管疾病]