黄蜀葵花中金丝桃苷的提取分离及纯度检测  

Preparation method of hyperoside from Flos Abelmoschus manihot

作　　者：肖顺丽 王敏[1] 李琦[1] 曹青云[1] 高荣凯[1] 张宏桂[1] 

机构地区：[1]北京中医药大学中药学院,100102

出　　处：《国际中医中药杂志》2017年第8期733-736,共4页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（30870262）

摘　　要：目的 研究黄蜀葵花中金丝桃苷的提取、分离、纯化方法.方法 采用聚酰胺柱层析、梯度洗脱方法提取分离黄蜀葵花中的金丝桃苷,以氢谱和碳谱鉴定其结构,以薄层色谱法、HPLC法进行纯度检查.结果 制得的金丝桃苷经薄层色谱法检查无杂质斑点,经HPLC法检测其纯度98.5%以上.结论 所建立的分离、纯化方法简便,成本低.Objective To study the preparation method and analytical technique of hyperoside from Flos Abelmoschus manihot.Methods Hyperoside was isolated and purified by solvent extract and chromatography, whose structure was determined by 1H-NMR and 13C-NMR. The purity was analyzed by TLC and HPLC.Results The TLC showed that the hyperoside had no impurity spot. The HPLC indicated that the purity reached more than 98.5%.Conclusions The mothod of isolation and purification for hyperoside reported in this paper was simple and economical.

关 键 词：金丝桃苷 提取法 分离和提纯 色谱法 高压液相 

分 类 号：R284.1[医药卫生—中药学]