2014—2015年江苏省肠道病毒71型VP1基因特征分析  

Analysis on genetic character of enterovirus 71 strains isolated from Jiangsu Province in 2014—2015

作　　者：单军[1] 夏瑜[2] 付建光[1] 王慎骄[1] 汤奋扬[1] 嵇红[1] 

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009 [2]苏州市疾病预防控制中心,江苏苏州215004

出　　处：《南京医科大学学报（自然科学版）》2017年第7期895-898,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金青年基金(81402732);江苏省预防医学课题(Y2012071);苏州市科技局应用基础研究课题(SYS201262)

摘　　要：目的:探讨2014—2015年江苏省肠道病毒71型(enterovirus 71,EV71)分离株的病毒基因特征。方法:用RD细胞对RT-PCR初筛EV71阳性的标本进行病毒分离,成功分离到的EV71分离株进行VP1区的序列测定和分析。序列分析结果用DNA Star和MEGA 6.0软件进行分析,并与各型参考株进行比较。结果:共分离到35株EV71型毒株,分离阳性率为29.17%。35株EV71分离株VP1区的核苷酸和氨基酸序列同源性分别为93.3%~100.0%和96.6%~100.0%,与EV71的C4a亚型代表株安徽阜阳株的核苷酸和氨基酸序列同源性分别为95.6%~97.9%和97.9%~100.0%;在系统进化树上与C4a亚型代表株相聚在一起,并且存在2个进化分支。结论:2014—2015年江苏省分离的35株EV71毒株均属于C4a亚型;江苏省EV71流行株可能与国内其他省市流行的病毒有着共同的祖先,再各自进化。Objective：To analyze the genic characteristic of enterovirus 7 I（EV71） strains isolated from Jiangsu Province in 2014-- 2015. Methods：The EVT1 RNA positive samples were inoculated into RD cells to separate the EV71 isolates,which were selected for sequencing and analysis of VP1 gene. The software DNA Star and MEGA 6.0 were used for comparison between EV71 and the vari- ous types of reference strains. Results：A total of 35 strains of EV71 were isolated and the positive rate was 29.17%. Sequence analy- sis of the 35 isolates showed that the homology of nucleotides and amino acids of VP1 gene were 93.3%-100.0% and 96.6%- 100.0%, respectively. There were 95.6%-97.9% nucleotide acid identity and 97.9%-100% amino acid identity among these 35 strains and representative strain of C4a genotype isolated from Fuyang City. Phylogenetic analysis showed that the 35 EVT1 strains gathered together with the representative strains of Cda genotype,and belonged to 2 viral transmission chains. Conclusion：The 35 EV71 strains from Jiangsu Province in 2014--2015 belong to C4a genotype. The major epidemic strain of EV71 in Jiangsu Province might have a same ancestor as domestic epidemic strains of EV71, and then evolve independently.

关 键 词：手足口病 肠道病毒71型 基因型 

分 类 号：R373.2[医药卫生—病原生物学]