高灵敏性表面增强拉曼散射传感器的构建及其用于人体尿液中痕量甲醛的快速检测  

Construction of highly sensitive surface-enhanced Raman scattering sensor and its application in the rapid detection for trace formaldehyde from human urine

作　　者：郑春辰 张丽颖[1] 都述虎[1] 

机构地区：[1]南京医科大学药学院,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2017年第7期906-911,914,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(61605084)

摘　　要：目的:构建响应快速、灵敏度高的表面增强拉曼散射(surface-enhanced Raman scattering,SERS)传感器,实现对人体尿液中痕量甲醛的快速检测。方法:以对-氨基苯硫酚(p-ATP)为探针分子,通过表面自组装技术在银纳米粒子表面修饰具有SERS信号响应的单分子层,滴加甲醛后,甲醛通过醛基与p-ATP发生特异性缩合反应,p-ATP原有拉曼信号强度猝灭,进而实现对甲醛的间接检测。结果:甲醛在1.0×10^(-8)~1.0×10^(-7) mol/L范围内呈现良好的线性,相关系数为0.991 7,检测限为6.5×10^(-9) mol/L,回收率为97.5%~106.0%。结论:本方法可用于人体尿液中痕量甲醛的检测,具有耗时少、简便和灵敏度高等优点。Objective：To construct highly sensitive surface-enhanced Raman scattering （SERS） sensor and implement the rapid detection of trace formaldehyde from human urine. Methods：A novel SERS sensor was fabricated by modifying p-aminothiophenol （p- ATP） on the surface of Ag nanopartieles （Ag NPs）. When the aqueous formaldehyde was dropped into Ag NPs colloid,the condensation reaction between p-ATP and formaldehyde resulted in the quenching of Raman signals of p-ATP. Thus,formaldehyde was indirectly detected by SERS. Results：Results showed that the method had a good linear relationship in the range of 1.0×10-8-1.0×10-7 mol/L with a correlation coefficient of 0.991 7. The limit of detection was 6.5×10-9 mol/L and the recoveries of formaldehyde were 97.5%-106.0% from the spiked urine samples,which were satisfactory. Conclusion：The method can be applied for the determination of trace formaldehyde in urine with the advantages of less time-consuming, non-preconcentrated procedure and high sensitivity.

关 键 词：甲醛 表面增强拉曼散射 对-氨基苯硫酚 银纳米粒子 尿液 

分 类 号：R446.12[医药卫生—诊断学]