电针“丰隆”穴对非酒精性脂肪肝大鼠肝组织固醇调节元件结合蛋白-1c的影响  

Effect of Electroacupunctrue Stimulation at “Fenglong”(ST 40) on Expression of SREBP-1 c in Non-alcoholic Fatty Liver Disease Rats

作　　者：余敏[1] 李钢[2] 唐成林[3] 高睿琦 冯启廷 曹净[3] 

机构地区：[1]重庆市北碚区中医院,重庆400700 [2]重庆市第九人民医院,重庆400700 [3]重庆医科大学中医药学院,重庆400016

出　　处：《针刺研究》2017年第4期308-314,共7页Acupuncture Research

基　　金：重庆市卫生计生委资助项目(No.AZY 201402078)

摘　　要：目的:观察电针"丰隆"穴对非酒精性脂肪肝大鼠肝组织固醇调节元件结合蛋白-1c(SREBP-1c)的影响,探讨电针治疗非酒精性脂肪肝的作用机制。方法:SD大鼠随机分为普食组、高脂对照组、手针丰隆组、电针丰隆组、电针足三里组,每组12只。除普食组外其余各组大鼠均用高脂饲料喂养复制非酒精性脂肪肝模型。手针丰隆组采用毫针针刺"丰隆",电针丰隆组及电针足三里组予以电针刺激双侧"丰隆"及"足三里"穴,每次20 min,每日1次,治疗4周。HE染色观察肝组织病理学改变,比色法检测血清游离脂肪酸(FFA)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的含量,反转录-聚合酶链反应、蛋白印迹法检测大鼠肝脏组织SREBP-1c基因与蛋白的表达。结果:HE染色结果显示,高脂对照组肝细胞排列紊乱,呈弥漫性大泡型脂肪空泡改变;手针丰隆组出现小泡型脂肪变性;电针丰隆组、电针足三里组肝细胞排列有序,脂肪变性减轻。高脂对照组大鼠血清FFA、ALT、AST含量及肝组织SREBP-1c基因与蛋白表达水平显著高于普食组(P<0.01);手针及电针各组血清FFA、ALT、AST含量及肝脏组织SREBP-1c基因与蛋白表达水平明显低于高脂对照组(P<0.01),且电针丰隆组较手针丰隆组降低更为明显(P<0.01,P<0.05)。电针丰隆组血清FFA含量及肝脏组织SREBP-1c基因与蛋白表达水平均低于电针足三里组(P<0.05)。结论:电针"丰隆""足三里"穴对非酒精性脂肪肝病大鼠有良性调节作用,其作用机制可能为下调SREBP-1c基因与蛋白表达,改善内质网应激,调节脂质代谢紊乱,从而减轻肝脏组织炎性损伤,且电针"丰隆"穴效果更优。Objective To observe the effect of electroacupuncture（EA）stimulation at＂Fenglong＂（ST 40）on expression of sterol regulatory element binding protein-1c（SREBP-1c）in non-alcoholic fatty liver disease（NAFLD）rats,so as to reveal its mechanism underlying improving NAFLD.Methods Male SD rats were randomly divided into normal diet group,high-fat diet group,ST 40 manual acupuncture（MA）group,ST 40 EA group and ＂Zusanli＂（ST 36）EA group（n=12in each group）.Apart from the normal diet group,rats in other groups were fed with high-fat diet to replicate NAFLD model.ST 40 MA group was treated with filiform needling for 20 min,EA（4Hz/20 Hz,5mA）was applied to bilateral acupoints of ST 40 and ST 36 respectively for 20 min in the ST 40 EA group and ST 36 EA group,once a day for 4 weeks.The pathological changes of hepatic tissue were observed by HE staining.The contents of serum free fatty acid（FFA）,alanine aminotransferase（ALT）and aspartate aminotransferase（AST）were detected by colorimetry.The expression of SREBP-1c mRNA and protein in the hepatic tissue were detected by reverse transcription-polymerase chain reaction（RT-PCR）and Western blot,respectively.Results HE staining showed that hepatocytes disorganized with diffuse bullous fat vacuolar changes in rats of the high-fat diet group,and small vesicle steatosis appeared in the ST 40 MA group.The liver cells arranged orderly and the steatosis decreased in the ST 40 EA group and ST 36 EA group.The contents of FFA,ALT,AST in serum and the expression levels of SREBP-1cmRNA and protein in the hepatic tissue were significantly higher in the high-fat diet group than in the normal diet group（P〈0.01）,while significantly lower in the MA and EA groups than in the high-fat diet group（P〈0.01）.Furthermore,the effects of treatment in the ST 40 EA group were better than those in the ST 40 MA group（P〈0.01,P〈0.05）.The FFA contents in serum and the expression levels of SREBP-1c mRNA and protein were decreased in the ST 40 EA gr

关 键 词：电针 非酒精性脂肪肝病 内质网应激 固醇调节元件结合蛋白-1C 

分 类 号：R245.97[医药卫生—针灸推拿学]