电针对糖尿病胃轻瘫大鼠胃窦组织胰岛素生长因子1及其受体的影响  

Effect of Electroacupuncture Intervention on Gastrointestinal Motility and Expression of Insulinlike Growth Factor 1 and Its Receptor Proteins in Gastric Antrum in Diabetic Gastroparesis Rats

作　　者：杨建文[1] 彭艳[2] 陈海交 张程程[1] 刘薇薇[1] 刘丽[1] 刘密[2] 林亚平[2] 

机构地区：[1]湖南中医药大学研究生院,长沙410208 [2]湖南中医药大学针灸推拿学院针灸生物信息分析重点实验室,长沙410208

出　　处：《针刺研究》2017年第4期315-320,共6页Acupuncture Research

基　　金：国家重点基础研究计划(No.2014CB 543102);国家自然科学基金项目(No.81403487);湖南省教育厅青年基金(No.14B128);湖南中医药大学研究生创新课题(No.2016CX 25)

摘　　要：目的:观察电针"足三里""梁门""三阴交"对糖尿病胃轻瘫(DGP)大鼠胃肠动力、胃窦胰岛素样生长因子1(IGF-1)及其受体(IGF-1R)表达的影响,探讨电针治疗DGP的可能机制。方法:SD大鼠随机分为空白对照组、模型对照组、电针腧穴组、电针非穴组、胃复安对照组,每组10只。采用腹腔注射2%链脲佐菌素(55mg/kg)结合高脂高糖不规律饮食制备DGP模型。电针腧穴组取单侧"足三里""梁门""三阴交"电针治疗,电针非穴组取同侧三穴对照点电针治疗,胃复安对照组予1.7%胃复安药液(1mL/100g)灌胃,均每天1次,连续干预15d。观察各组大鼠症状积分、血糖、胃排空率、小肠推进率,ELISA法检测胃窦组织IGF-1及IGF-1R的含量。结果:与空白对照组比较,模型对照组胃排空率及小肠推进率明显降低,血糖、症状积分、胃窦组织IGF-1及IGF-1R含量明显升高(P<0.01)。与模型对照组比较,电针腧穴组、胃复安对照组胃排空率及小肠推进率明显升高(P<0.05,P<0.01),电针腧穴组血糖、症状积分、胃窦组织IGF-1及IGF-1R含量明显降低(P<0.05,P<0.01)。与电针腧穴组比较,电针非穴组及胃复安对照组症状积分、胃窦组织IGF-1及IGF-1R含量均明显升高(P<0.05,P<0.01)。结论:电针腧穴能降低DGP大鼠血糖水平,促进胃肠动力,其作用机制可能与降低胃窦组织IGF-1及IGF-1R含量有关。Objective To observe the effect of electroacupuncture（EA）intervention of＂Zusanli＂（ST 36）,etc.on gastrointestinal motility and levels of insulin-like growth factor 1（IGF-1）and IGF-1receptor（IGF-1R）proteins in the gastric antrum in diabetic gastroparesis（DGP）rats,so as to study its mechanism underlying improvement of DGP.Methods Fifty SD rats,half male and half female,were randomly allocated to control,model,EA acupoint,EA non-acupoint and medication（metoclopramide）groups（n=10rats/group）.The DGP model was induced by 2% streptozotocin（STZ,i.p.i.,55mg/kg）and high fatsugar forage for 8weeks.EA（10Hz/50 Hz,2mA）was applied to＂Zusanli＂（ST 36）,＂Liangmen＂（ST 21）,＂Sanyinjiao＂（SP 6）and non-acupoints（about 5mm lateral to ST 36,ST 21 and SP 6,respectively）for 20 min,one daily for 15 days,and 1.7% metocloppramide（1mL/100g）was given（gavage）to rats of the medication group,once daily for 15 days.After the treatment,the rats＇ general conditions were scored,the blood glucose level was determined using aglucometer,and the gastrointestinal mobility was evaluated by measuring the gastric emptying rate（GER）and the intestinal propulsion rates（IPR）marked by oral-infused phenol red solution（50mg/dL）.The immunoactivity levels of IGF-1and IGF-1Rof gastric antrum tissue were detected by enzymelinked immunosorbent assay（ELISA）.Results After modeling,the symptom integrative score,the blood glucose,IGF-1and IGF-1Rlevels were significantly increased（P〈0.01）,and the GER and IPR were notably decreased relevant to the control group（P〈0.01）.After EA stimulation of ST 36,ST 21 and SP 6,the symptom score,blood glucose,and IGF-1 and IGF-1R levels of the gastric antrum were significantly reduced,while the GER and IPR were considerably increased（P〈0.05,P〈0.01）.Except the GER and IPR were considerably increased in the medication group（P〈0.05,P〈0.01）,no significant changes were found in the symptom integrative score,the blood glucose,IGF-

关 键 词：电针 糖尿病胃轻瘫 胃动力 胰岛素样生长因子1 胰岛素样生长因子1受体 胃窦 

分 类 号：R245.97[医药卫生—针灸推拿学]