Efficacy of Photogem（Hematoporphyrin Derivative） as a Photoactivatable Larvicide against Aedes aegypti （Diptera： Culicidae） Larvae  

作　　者：Larissa Marila de Souza Natalia Mayumi Inada Sebastiao Pratavieira Juliano Jose Corbi Cristina Kurachi Vanderlei Salvador Bagnato 

机构地区：[1]Federal University of S6o Carlos, PPG Biotec, S6o Carlos 13565-905, Sao Paulo, Brazil [2]Sao Carlos Institute of Physics, University of Sao Paulo, Sao Carlos 13560-970, Sao Paulo, Brazil [3]Sao Carlos School of Engineering, University of Sao Paulo, Sao Carlos 13560-970, Sao Paulo, Brazil

出　　处：《Journal of Life Sciences》2017年第2期74-81,共8页生命科学（英文版）

摘　　要：Dengue vector is responsible for millions of deaths every year and has caused disastrous impacts on health systems. The continuous use of chemical insecticides, such as carbamates, pyrethroids and organophosphates generates resistant populations of the mosquito, therefore, new control methods must be investigated. The joint action of the population and guidelines for preventing the reproduction of the mosquito associated with the use of photoactivatable insecticides can be the alternative for the control of epidemiological outbreaks in affected regions. In this study, the photo-larvicidal activity of Photogem^（PG）, a derivative of hematoporphyrin, was investigated against 2nd-early 3rd instar of Aedes aegypti larvae （Diptera： Culicidae） under different lighting conditions （artificial lighting system and sunlight）. The dynamics of PG accumulation was characterized by CLSM （confocal laser scanning microscopy） and total time PG eliminationin solution was investigated by ultraviolet-visible spectrophotometry. The maximum photo-activity of PG was observed in 0.5 h under sunlight exposure which achieved 100% larval mortality. Fluorescence images showed a uniform distribution of PG along the digestive tract. PG remained stable in the sunlight for 48 h and in an artificial lighting system for longer periods, therefore, it can be used for the control ofAedes aegypti larvae as a new alternative to chemical insecticides. The method is considered environmentally friendly due to its rapid degradation in the presence of light. Further studies are required, so that the potential of the technique can be explored in real breeding places.

关 键 词：Photoactivatable larvicide PG hematoporphyrin derivative Aedes aegypti natural lighting artificial lighting. 

分 类 号：TQ453.291[化学工程—农药化工] Q969.453.8[生物学—昆虫学]