通过聚合酶链式反应研究稀土离子对基因序列的体外诱变作用  

The Mutagenic Effeet of Rare Earth Ions on a Gene Sequence in Vitro by Polymerase Chain Reaction

作　　者：李文彬[1] 盖晓静 王美娟[1] 刘丽华[1] 

机构地区：[1]内蒙古工业大学化工学院,内蒙古呼和浩特010051

出　　处：《稀土》2017年第4期142-147,共6页Chinese Rare Earths

基　　金：内蒙古自然科学基金项目(2017MS(LH)0302);内蒙古工业大学科学研究项目(ZD201208)

摘　　要：通过聚合酶链式反应(PCR)研究稀土离子对基因序列的体外诱变作用。以克隆在质粒上的乙酰化酶基因做模板进行PCR,在反应体系中加入不同浓度的稀土离子,并将PCR产物克隆测序。稀土离子对PCR有影响,当稀土离子的浓度达到1×10^(-3)mol/L时可以抑制PCR;将诱变后的乙酰化酶基因序列与原序列对比发现,稀土离子可以在体外通过PCR引起基因突变。突变是点突变即由T变为C和由G变为A的转换。The aim of this research is to study the mutagenic effeet of rare earth ions on a gene sequencn. The plasmid containing the acetylation of enzyme gene was used as a template for PCR , different concentrations of rare earth ions were add- ed into the reaction system,the PCR products were cloned and sequenced. Rare earth ions can influence the PCR and inhibit the PCR when the concentration of rare earth ions reaches 1 × 10^-3mol/L. The obtained sequences were aligned with the origi- nal gene sequences,the results show that rare earth ions can cause gene mutations in vitro by PCR. The mutation is a point mutation - a single base change. The most mutations are transitions（e, g. GC→TA）.

关 键 词：聚合酶链式反应 稀土离子 乙酰化酶基因 

分 类 号：TQ424[化学工程]