新疆哈萨克族高血压病患者淋巴细胞KCa3.1及CaN-NFAT信号通路的变化  

Change of lymphocyte KCa3.1 and CaN-NFAT signaling pathway in Kazak hypertensive patients from Xinjiang

作　　者：王凌鹏[1] 赵丽华[2] 刘娟[3] 曹雷雨[3] 罗健[3] 

机构地区：[1]新疆医科大学第一附属医院心脏中心,新疆乌鲁木齐830054 [2]新疆维吾尔自治区公安消防总队门诊部 [3]新疆医科大学第一附属医院内科VIP中心,新疆乌鲁木齐830054

出　　处：《心血管康复医学杂志》2017年第4期355-359,共5页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：新疆维吾尔自治区自然科学基金资助项目(2014211C077)~~

摘　　要：目的:研究新疆高高敏C反应蛋白(hsCRP)水平哈萨克族高血压患者外周血淋巴细胞钙调神经磷酸酶/活化T细胞核因子(CaN/NFAT)信号通路及其钙激活钾通道(KCa3.1)表达发生的变化,探讨淋巴细胞钾通道参与高血压病炎症反应的机制。方法:选择2014年2月至2014年11月在我院心脏中心首诊,未经降压治疗的新疆哈萨克族高血压患者。根据检测的hsCRP水平,选择hsCRP水平≥3mg/L的患者50例为高hsCRP组,hsCRP水平<3mg/L的患者50例为正常hsCRP组。检测比较两组KCa3.1、肿瘤坏死因子(TNF)-α基因、蛋白表达水平,以及淋巴细胞CaN、NFAT活性。结果:与正常hsCRP组比较,高hsCRP组淋巴细胞KCa3.1mRNA[(0.86±0.16)比(2.48±0.22)]、TNF-αmRNA[(1.02±0.15)比(2.90±0.13)]、KCa3.1蛋白[(1.00±0.02)比(2.46±0.04)]、TNF-α蛋白[(1.01±0.02)比(2.04±0.06)]表达水平、CaN活性[(1.04±0.15)比(2.83±0.08)]、NFAT活性[(0.96±0.06)比(2.65±0.07)]均显著升高,P均<0.01。结论:具有炎症反应的新疆哈萨克族高血压患者外周血淋巴细胞上KCa3.1及CaN-NFAT信号通路表达增加,提示淋巴细胞KCa3.1可能通过CaN/NFAT信号通路参与高血压病的发生发展。Objective： To study changes of peripheral blood lymphocyte calcineurin/nuclear factor of activated T cells （CaN/NFAT） signaling pathway and calcium activated potassium channel （Kca3.1） expression in Kazak hyperten- sive patients with high high sensitive C reactive protein （hsCRP） level from Xinjiang, and explore the mechanism of lymphocyte potassium channel involving in inflammation of hypertension. Methods： Kazak hypertensive patients from Xinjiang, who were first diagnosed in our heart center without antihypertensive therapy from Feb 2014 to Nov 2014, were selected. According to detected hsCRP level, a total of 50 hsCRP≥3mg/L patients were enrolled as high hsCRP group, and 50 hsCRP〈3mg/L patients were enrolled as normal hsCRP group. Gene and protein expression levels of Kca3.1 and tumor necrosis factor （TNF） -a, and activities of lymphocyte CaN and NFAT were measured and compared between two groups. Results： Compared with normal hsCRP group, there were significant rise in expression levels of lymphocyte Kc,3.1 mRNA [- （0.86±0.16） vs. （2.48±0.22）], TNF-a mRNA [ （1.02±0.15） vs. （2.90±0.13）], Kc,3.1 protein [- （1.00 ±0.02） vs. （2.46±0.04）] and TNF-a protein [ （1.01 ± 0.02） vs. （2.04 ± 0.06）], and activities of CaN [ （1.04 ± 0.15） vs. （2.83 ± 0.08）] and NFAT [- （0.96 ± 0.06） vs. （2.65 ± 0.07）] in high hsCRP group, P〈0.01 all. Conclusion： In Kazak hypertensive patients with inflammatory reaction from Xinjiang, Kca3.1 and CaN-NFAT signaling pathway expression rises, suggesting that lymphocyte Kca 3.1 may be involved in occurrence and development of hypertension via CaN/NFAT signaling pathway.

关 键 词：新疆 高血压 钾通道 

分 类 号：R544.109[医药卫生—心血管疾病]