磁性纳米粒子的制备及在转基因大豆检测中的应用  

Preparation of magnetic nanoparticles for genetically modified soybean detection

作　　者：赵晓丽[1] 梁新苗[1] 槐硕 边勇[1] 孙宁[2] 邓丛良[1] 田婧[3] 

机构地区：[1]北京出入境检验检疫局植物检疫中心,北京100026 [2]东南大学 [3]农业部规划设计研究院

出　　处：《植物检疫》2017年第4期31-33,共3页Plant Quarantine

基　　金：国家质检总局科技计划项目(2015IK020)

摘　　要：利用热分解法和表面4-羧基苯硼酸修饰,制备了用于转基因大豆提取基因组DNA的纳米粒子。制备的纳米粒子分散性好,磁响应性高。对比磁性纳米粒子和植物基因组DNA提取试剂盒提取转基因大豆基因组DNA的提取效果,结果表明,磁性纳米粒子提取转基因大豆基因组DNA的浓度明显高于试剂盒,实时荧光PCR检测转基因大豆的Ct值低于植物基因组提取试剂盒。Magnetic nanoparticles used to extract nucleic acid from genetically modified soybean were synthesized by thermal decomposition and modified with 4-Carboxyphenylboronic acid（CPBA） on surface.The magnetic nanoparticles have good monodispersed property and high magnetic responsive property.Compared the DNA extraction effect between magnetic nanoparticles method and plant genomic DNA kit method,the results showed that the concentration of DNA extracted by magnetic nanoparticles method was significantly higher than the kit method,the Ct value of nucleic acid extraction magnetic nanoparticles method was lower than that of plant genomic DNA kit method.

关 键 词：纳米粒子 4-羧基苯硼酸 DNA提取 转基因大豆 

分 类 号：S565.1[农业科学—作物学]