油菜茎基溃疡病菌DPO-PCR检测方法的建立  

Development of DPO-PCR method for the detection of Leptosphaeria maculans

作　　者：龙阳 袁俊杰 候翠丽 卢乃会 杨卓瑜 马新华 魏霜[3] 

机构地区：[1]湛江出入境检验检疫局检验检疫技术中心,广东湛江524000 [2]中国检验认证集团广东有限公司湛江分公司 [3]广东出入境检验检疫局检验检疫技术中心

出　　处：《植物检疫》2017年第4期42-45,共4页Plant Quarantine

基　　金：国家质检总局科技项目(2015IK080);广东出入境检验检疫局科技计划项目(2016GDK32)

摘　　要：根据油菜茎基溃疡病菌ITS基因序列,设计特异性DPO引物,建立检测油菜茎基溃疡病菌的DPO-PCR检测方法,并对其特异性、灵敏性进行评价。结果显示与常规PCR检测方法相比,DPO-PCR反应对退火温度不敏感,具有更强的特异性。利用该方法对10批油菜籽样品进行检测,共检出5批阳性样品,检测结果与常规PCR一致,为油菜茎基溃疡病菌的检测提供了新方法。A pairs of DPO primers was designed based on ITS sequences of Leptosphaeria maculans. A DPO-PCR method was developed to detect L. maculans,and the specificity and sensitivity of the method were tested. The result showed that DPO-PCR method insensitivity to annealing temperature and have a higher specificity than conventional PCR. A total 5 positive samples for Leptosphaeria maculans were detected from 10 samples by the DPO-PCR method,which was in accordance with the result by conventional PCR. DPO-PCR provided a new method to detect Leptosphaeria maculans.

关 键 词：油菜茎基溃疡病菌 DPO-PCR 检测 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]