人参皂苷Rg1对β淀粉样蛋白诱导的神经细胞凋亡的研究  

Effect of Ginsenoside Rg1 on β-amyloid Induced Neuron Apoptosis

作　　者：叶茂盛[1] 赵东杰[1] 周勇[1] 蒋蓝英[1] 

机构地区：[1]浙江省中西医结合医院老年病科,杭州310003

出　　处：《浙江中西医结合杂志》2017年第8期642-645,F0002,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2010ZB112)

摘　　要：目的探讨人参皂苷Rg1对大鼠海马组织β淀粉样蛋白(Aβ)诱导的神经细胞凋亡的影响。方法 36只SD雄性大鼠分为假手术组、Aβ模型组和人参皂苷Rg1干预组。Aβ模型组和人参皂苷干预组直接注射10μg/μL的β淀粉样蛋白1-42(Aβ1-42)1μL于大鼠海马组织造模;人参皂苷干预组大鼠术后用人参皂苷Rg1 50mg/kg连续灌胃30天。采用Morris水迷宫测试大鼠学习记忆能力,甲醇刚果红染色观察人参皂苷Rg1对海马组织Aβ的沉积影响,TUNEL法检测海马神经细胞凋亡。结果假手术组大鼠水迷宫测试逃避潜伏时间为(47.36±12.21)s,穿越平台次数为(7.48±1.25)次。与假手术组比较,Aβ模型组大鼠水迷宫测试逃避潜伏时间延长至(84.52±16.68)s(P<0.01),穿越平台次数减少至(2.57±0.64)次(P<0.01)。与Aβ模型组比较,人参皂苷干预组大鼠的逃避潜伏时间[(63.22±12.05)s,P<0.05]和穿越平台次数[(5.22±1.31)次,P<0.05]均显著改善。Aβ模型组大鼠海马组织出现明显Aβ沉积;人参皂苷干预组大鼠海马组织Aβ沉积的阳性斑块数量少于Aβ模型组。人参皂苷干预组海马组织TUNEL阳性神经细胞数目比Aβ模型组明显减少[(73.20±1.71)个比(101.83±1.45)个,P<0.01],Aβ模型组海马组织细胞出现明显的细胞凋亡。结论人参皂苷Rg1可明显改善Aβ所致AD大鼠学习记忆功能,减少海马组织的Aβ沉积,抑制大鼠海马Aβ诱导的细胞凋亡。Objective To investigate the effect of ginsenoside Rg1 on β-amyloid（Aβ） inducedneuron apoptosis in the hippocampusarea. Methods Thirty-six Sprague-Dawley rats were assigned into 3 groups. Rats in the shamoperated group were injected with normal saline. Rats in the Aβ group and ginsenoside Rg1 group were injected with 1μL Aβ1-42 at the concentration of 10μg/μL. Rats in the ginsenoside Rg1 group were fed with ginsenoside Rg1 solution of 50mg/kg for 30 days consecutively. The Morris water maze was used to assess the ability of learning and memory in rats. The effect of Aβ and ginsenoside Rg1 on the hippocampus cells was observed by Congo red staining of methanol.The apoptotic neurons were detected by TUNEL method. Results The escape latency of rats was（47.36±12.21）s and and the number of rats crossing platform was（7.48±1.25） in the sham-operated group,which increased to 84.52±16.68 s and and 2.57±0.64 in the Aβ group respectively.Compared with Aβ group,the escape latency（63.22 ±12.05）s of rats was decreasedand the number of rats crossing platform（5.22 ±1.31） was increased in the ginsenoside Rg1 groupsignificantly（bothP〈0.05）. The Aβ peptide deposition was found in the hippocampus ofrats. More positive staining materials aggregated in the Aβ group compared with the ginsenoside Rg1 group by Congo red staining. The apoptotic neurons inthe hippocampus of rats in Aβ injection group were increased significantly.The number of TUNEL positive neuron in the hippocampus of ginsenoside Rg1 group wasfewer than that in the Aβ group（73.20±1.71 vs 101.83±1.45,P〈0.01）. Conclusion Ginsenoside Rg1 can reduce the deposition of Aβ in the hippocampus and improve the learning and memoryfunction of rats by reducing the Aβ-induced apoptosis in the hippocampus.

关 键 词：大鼠 阿尔茨海默病 海马组织 人参皂苷 Β-淀粉样蛋白 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]