加味酸枣仁汤对睡眠剥夺大鼠细胞免疫功能紊乱的调节作用  

Regulation of Jiawei Suanzaoren Decoction to Cellular Immune Dysfunction Caused by Sleep Deprivationin Rats

作　　者：刘文娟[1] 胡霖霖[1] 刘义[1] 宋明芬[2] 蒋海寅[3] 张永华[4] 

机构地区：[1]杭州市第七人民医院心身科,杭州310013 [2]杭州市第七人民医院分子生物学研究室,杭州310013 [3]浙江大学附属第一医院传染病诊治重点实验室,杭州310006 [4]杭州市第七人民医院睡眠障碍诊疗中心,杭州310013

出　　处：《浙江中西医结合杂志》2017年第8期659-661,668,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：杭州市卫生计生科技计划项目(No.2016A47)

摘　　要：目的观察加味酸枣仁汤对睡眠剥夺所致大鼠细胞免疫功能紊乱的调节作用。方法选择SPF级SD大鼠40只,随机分为正常组、模型组、西药组、中药组各10只。正常组大鼠给予无干扰常规喂养7天;余三组均采用平台随机移动法造模7天,造模开始模型组同时予生理盐水灌胃、西药组予劳拉西泮片0.105mg/(kg·d)、中药组予加味酸枣仁汤6.3g/(kg·d)灌胃,共7天。测量各组大鼠体质量,通过旷场实验进行行为学评定,测定静脉血中CD4+、CD8+、CD4+/CD8+,以及血清中白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、IL-2/IL-6水平。结果与正常组比较,模型组大鼠体质量显著下降[(205±12.11)g比(254±13.33)g,P<0.01],旷场实验水平与垂直得分显著升高[(66.64±11.82)分比(44.71±13.82)分,(26.43±4.82)分比(16.38±5.45)分,P<0.01),CD4+/CD8+、IL2/IL6明显下降[(1.16±0.64)比(1.74±0.52),(0.13±0.14)比(0.35±0.28),P<0.05]。与模型组比较,中药组体质量明显升高[(219±14.45)g比(205±12.11)g,P<0.05],但和西药组比较无明显差异[(219±14.45)g比(217±13.23)g,P>0.05)],旷场实验水平和垂直得分明显下降[(51.35±13.37)分比(66.64±11.82)分,(21.73±4.39)分比(26.43±4.82)分,P<0.05],但明显高于西药组[(51.35±13.37)分比(46.12±14.87)分,(21.73±4.39)分比(15.83±5.42)分,P<0.05];CD4+/CD8+、IL2/IL6比值明显升高[(2.26±0.65)比(1.16±0.64),(0.29±0.19)比(0.13±0.14),P<0.01],并明显高于西药组[(2.26±0.65)比(1.71±0.71),(0.29±0.19)比(0.17±0.11),P<0.01]。结论加味酸枣仁汤能改善睡眠剥夺导致的模型大鼠体质量下降和行为学改变,并能纠正其细胞免疫功能紊乱。Objective Toobserve the regulation of Jiawei Suanzaoren decoction for cellular immune dysfunction in rats with sleep deprivation. Methods Forty SD rats was randomly divided into four groups： control group, model group, western medicine group and Chinese medicine group. Control group was given normal feeding. Exceptcontrolgroup,others all used BW-NSD404 sleepd eprivationmachineto make model for 7 days. Model group wereadministered intragastrically with normal saline（NS）, western medicine group with lorazepam tablets（0.105mg/（kg·d））, chinese medicine group with Jiawei Suanzaoren decoction（6.3g/（kg·d）） every day. The weight of rats were measured,CD4＋, CD8＋, CD4＋/CD8＋ invein,was determined and serum IL-2, IL-6, and IL-2/IL-6 was detected.Open Field Test（OFT） was used to evaluate the behavior of rats. Results Compared with control group, the weight of rats in the model group reduced（205 ±12.11 g vs 254 ±13.33g; P〈0.01）, the horizontal and vertical score raised in OFT（66.64±11.82 vs 44.71±13.82; 26.43±4.82 vs 16.38±5.45;P〈0.01）, CD4＋/CD8＋ and IL-2/IL-6 ratios were lower（1.16±0.64 vs 1.74±0.52; 0.13±0.14 vs 0.35±0.28;P〈0.05）. Compared with model group, chinese medicine group had increasedbody weight（219±14.45,P〈0.05）, but no significant difference to that of western medicine group（217±13.23,P〈0.05）, lower horizontal and vertical scores of OFT（51.35±13.37, 21.73±4.39,P〈0.05）,but higher than that of western medicine group（46.12±14.87, 15.83±5.42,P〈0.05）, higher CD4＋/CD8＋ and IL-2/IL-6 ratios（2.26±0.65,0.29±0.19,P〈0.01）,and also higher than that of western medicine group（1.71±0.71, 0.17±0.11,P〈0.01）. Conclusion Jiawei Suanzaoren decoction can improve the weight of rats with sleep deprivation and correct their behavior as well as adjusting the immune function disorder.

关 键 词：大鼠 睡眠剥夺 加味酸枣仁汤 细胞免疫 

分 类 号：R285.5[医药卫生—中药学]