Ghrelin对糖尿病大鼠心肌动作电位和瞬时外向钾电流的影响  

Effects of ghrelin on action potential and transient outward potasium current in streptozotocin-induced diabetic cardiomyopathy rats

作　　者：冯丹阳[1,2] 孙强[1] 陈莉娜[1] 赵正杭[1] 周筠[1] FENG Da n-yang SUN Qiang CHEN Li-na ZHAO Zheng-hang ZHOU Jun(Department of Pharmacology, School of Basic Medical Sciences of Xi＇an Jiaotong University Health Science Center, Xi＇an 710061 Department of Pharmacy, Xi＇an Electric Central Hospital, Xi＇an 710032, China)

机构地区：[1]西安交通大学医学部基础医学院药理学系,陕西西安710061 [2]西安市电力中心医院药剂科,陕西西安710032

出　　处：《西安交通大学学报（医学版）》2017年第5期661-664,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金面上项目(No.81170176);陕西省科技统筹创新工程计划项目(No.2014KTCL03-10)~~

摘　　要：目的探讨外源性ghrelin对糖尿病心肌病(DCM)大鼠心肌动作电位及瞬时外向钾电流(Ito)的影响。方法雄性SD大鼠腹腔注射链脲佐菌素(60mg/kg)诱导Ⅰ型DCM模型,并采用全细胞膜片钳技术观察ghrelin对糖尿病大鼠心室肌细胞动作电位及瞬时外向钾电流的影响。结果与DCM组比较,10^(-7) mmol/L ghrelin明显延长DCM大鼠心室肌细胞5%动作电位时程(APD50)[(12.49±2.32)%;n=7,P=0.037]及APD90[(26.29±5.13)%;n=7,P=0.006];并明显抑制Ito电流峰值,抑制率[(23.14±3.07)%;n=9,P=0.021]。结论外源性ghrelin抑制Ito电流而延长DCM大鼠心肌动作电位时程,参与糖尿病大鼠心脏电生理的重构。Objective To study the effects of ghrelin on action potential （AP） and transient outside K^＋ current （I,o） in ventricular myocytes of diabetic cardiomyopathy （DCM） rats. Methods A rat model of DCM was established by single intraperitoneal injection of streptozotocin （STZ, 60 mg/kg）, and whole cell patch-clamp technique was used to record the effects of 10.7 mmol/L ghrelin on AP and /to current density in the DCM rat ventricular myocytes. Results Compared to DCM group, Ghrelin of 10.7 mmol/L could significantly prolong APDs0[（12.49±2.32）%; n=7, P=0.037] and APD90[（26.29±5.13）%; n=7, P=0.006] and decrease /to current peak value [（23. 14± 3.07）% ; n = 9, P= 0. 021] in DCM rat ventricular myocytes. Conclusion Exogenous ghrelin is involved in the electrophysiological reconstruction of the heart of diabetic rats by decreasing /to current and prolonging APD in STZ-induced DCM rat ventricular myocytes.

关 键 词：GHRELIN 糖尿病心肌病 动作电位 瞬时外向钾电流 

分 类 号：R318.11[医药卫生—生物医学工程]