高血压病患者外周血淋巴细胞ROS水平变化与血压昼夜节律的关系  

Relationship between ROS level of peripheral blood lymphocytes and blood pressure circadian rhythm in patients with hypertension

作　　者：艾文伟[1] 魏伟荣[1] 张明[2] 雷梦觉[1] 胡杰[1] 

机构地区：[1]江西省人民医院二部心内二科,江西南昌330006 [2]江西省人民医院二部心内一科,江西南昌330006

出　　处：《中国临床研究》2017年第8期1026-1029,1034,共5页Chinese Journal of Clinical Research

基　　金：江西省卫生厅科技计划项目(20143028)

摘　　要：目的探讨原发性高血压病患者外周血淋巴细胞活性氧自由基(ROS)水平变化与血压昼夜节律的关系。方法选择2014年5月至2016年1月62例原发性高血压患者作为研究对象,行24 h动态血压监测,记录24 h平均收缩压及舒张压、白天平均收缩压及舒张压、夜间平均收缩压及舒张压等,按照血压昼夜变化分为杓型血压组(n=21)、非杓型血压组(n=24)及反杓型血压组(n=17),应用二氯荧光黄双乙酸盐(DCFH-DA)探针标记外周血淋巴细胞ROS,采用流式细胞术检测ROS水平[脱氧助间型霉素(DCF)平均荧光强度]。同时以18例同期进行健康体检血压正常的成年人作为对照。结果高血压患者包括杓型血压组、非杓型血压组及反杓型血压组淋巴细胞ROS水平较对照组均显著增加(P均<0.05),其DCF荧光强度分别为:杓型血压组546.35±30.29,非杓型血压组567.63±35.35,反杓型血压组591.68±38.57,两两比较均有统计学差异(P均<0.05)。相关性分析显示淋巴细胞ROS水平与夜间平均收缩压及夜间平均舒张压呈显著正相关(r=0.451,r=0.425,P均<0.05),与白天平均舒张压呈负相关(r=-0.218,P<0.05)。结论高血压患者外周血淋巴细胞ROS水平增加,且与杓型高血压患者比较,非杓型和反杓型高血压患者淋巴细胞ROS水平进一步增加,且夜间血压的升高与患者外周血淋巴细胞ROS水平增加相关,提示淋巴细胞ROS改变与血压昼夜异常相关,。Objective To investigate the relationship between reactive oxygen species（ ROS） level of peripheral blood lymphocytes and blood pressure circadian rhythm in patients with essential hypertension. Methods A total of 62 patients with primary hypertension admitted to cardiology between May 2014 and January 2016 were selected as research objects.Ambulatory blood pressure monitoring was performed in all patients to record 24 h mean systolic blood pressure（ SBP） and mean diastolic blood pressure（ DBP）,daytime mean SBP and mean DBP,nighttime mean SBP and mean. The hypertension patients were divided into groups dipper（ n = 21）,non-dipper（ n = 24） and anti-dipper（ n = 17） according to the circadian changes blood pressure. After using 2 ＇,7 ＇ dichlorofluorescein diacetate（ DCFH-DA） probe to label the ROS of peripheral blood lymphocytes,flow cytometry was used to detect ROS levels of lymphocytes expressing with deoxycoformycin（ DCF）mean flourscence indensity. Eighteen healthy volunteers were selected as control group. Results Compared with control group,the ROS levels of lymphocytes were significantly increased in hypertension group including dipper group,non-dipper group and anti-dipper group（ all P〈0. 05） in which DCF fluorescence intensity were 546. 35 ± 30. 29 in dipper group,567. 63 ± 35. 35 in non-dipper group,591. 68 ± 38. 57 in anti-dipper group,and there were significant differences in any pairwise comparison（ all P〈0. 05）. Correlation analysis showed that ROS level of peripheral blood lymphocytes was positively correlated with nighttime mean SBP and nighttime mean DBP（ r = 0. 451,r = 0. 425,all P〈0. 05） and negatively correlated with daytime mean DBP（ r =-0. 218,P〈0. 05）. Conclusion ROS level of peripheral blood lymphocytes increases in patients with hypertension,and further increases in non-dipper group and anti-dipper group compared with dipper group. The elevation of nighttime blood was closely associated with the increase of ROS levels of peripheral

关 键 词：高血压病 活性氧自由基 淋巴细胞 血压昼夜节律 杓型血压 非杓型血压 反杓型血压 

分 类 号：R544.1[医药卫生—心血管疾病] R446.11[医药卫生—内科学]