利用献血者捐献血液中白细胞制备的异体CIK细胞与肿瘤靶细胞体外杀伤试验研究  

Study on in vitro killing test of CIK cells and allogeneic tumor target cells prepared from whole blood leukocytes of donors

作　　者：范恩勇 郭铭华 孙海英 黄文杰 焦玉东 

机构地区：[1]扬州市中心血站、扬州市输血研究所,江苏扬州225007

出　　处：《临床血液学杂志（输血与检验）》2017年第2期258-260,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：江苏省采供血行业开放课题(No:JSCGX-201508)

摘　　要：目的:探讨利用献血者捐献血液中的白细胞制备的异体细胞因子诱导的杀伤(CIK)细胞与肿瘤靶细胞体外杀伤试验情况。方法:重悬献血者CIK细胞与肝癌细胞、人乳腺癌细胞、白血病细胞、B淋巴瘤细胞浓度,以CIK细胞为效应细胞,肿瘤细胞为靶细胞,使效靶比为32∶1、16∶1、8∶1、4∶1、2∶1、1∶1时共培养48h后检测450nm下的吸收度A值。结果:献血者异体CIK细胞对肝癌、乳腺癌、白血病、B淋巴瘤细胞4种肿瘤靶细胞的杀伤率随着效应细胞数的增加而增大,当效靶比为32∶1时,献血者异体CIK细胞的杀伤率分别为86.1%、84.3%、62.5%、87.9%;献血者异体CIK细胞对肝癌、乳腺癌、B淋巴瘤细胞杀伤活性明显强于白血病细胞,同一效靶比时,CIK细胞对肝癌细胞杀伤活性较高,对白血病细胞杀伤活性较差。结论:献血者CIK细胞对肝癌、人乳腺癌、白血病、B淋巴瘤细胞等肿瘤细胞的生长均有明显的抑制作用。4种肿瘤靶细胞的杀伤率随着效应细胞数的增加而增大,同一效靶比时,CIK细胞对肝癌细胞杀伤活性较高,对白血病细胞杀伤活性较差。Objective： To investigate in vitro killing test of CIK cells and tumor killing target cells prepared from donors＇ blood leukocytes. Method： Donor CIK cells were resuspended with hepatoma cells, human breast cancer cells, leukemia cells, B-cell lymphoma. In the mixture, CIK cells were as effector cells and tumor cells as target cells, the efficiency to target ratios were 32： 1, 16： 1,8： 1,4： 1,2：1 and 1： 1, then co-cultured 48 h, then detected A value at 450 nm. Result： The killing rates of donor allogeneic CIK cells to liver cancer, breast cancer, leukemia and B lymphoma cell increased with the increasing of the number of effector cells. When the efficiency to target ratio was 32： 1, the killing rates of donor allogeneic CIK cells were respectively 86.1%, 84.3%, 62.5% and 87.9%. The killing activity of donor allogeneic CIK cells on liver, breast, B lymphoma cell was stronger than that of leukemia cell. With the same efficiency to target ratio, the killing activity of CIK cells on hepatoma cells was high, while on leukemic cell was poor. Conclusion： Donor allogeneic CIK cells might have significant inhibi- tion on the growth of liver cancer, human breast cancer, leukemia, B lymphoma cells and other tumor cells. The killing rates of the CIK cells increased with the increasing of the number of effector cells. With the same efficiency to target ratio, the killing activity of CIK cells on hepatoma cells was high, while on leukemic cell was poor.

关 键 词：无偿献血 CIK细胞 肿瘤细胞 细胞杀伤试验 

分 类 号：R457.1[医药卫生—治疗学]