白头翁汤加减灌肠方对溃疡性结肠炎肠黏膜NF-κB mRNA的影响  

Therapeutic Observation of Pulsatilla Enema Treatment on the Ulcerative Colitis and the Expression of NF-κB Mrna in Mucosa Cells

作　　者：戴高中[1] 陈晨 范先靖[1] 田秋实[1] 周春刚[1] 朱世楷[1] 

机构地区：[1]无锡市中医医院,江苏无锡214071 [2]常州武进中医医院,江苏常州213161

出　　处：《中国中医基础医学杂志》2017年第7期970-972,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：无锡市科技局2011年无锡市第9批科技发展计划项目(CSE01N1101)-地方名中医治疗方案可持续利用的传承研究与应用示范

摘　　要：目的:使用白头翁汤加减灌肠方治疗急性期左半结肠型溃疡性结肠炎患者,观察其疗效及对肠黏膜NF-κB mRNA表达的影响并探讨其机制。方法:34例左半结肠型患者随机分组法分为2组,治疗组采用白头翁汤加减灌肠方,对照组给予SASP保留灌肠,疗程均为45 d,比较2组的临床疗效及结肠镜下表现,RT-PCR法测定肠黏膜NF-κB mRNA的表达。结果:2组治疗后各症状均较前好转,其中治疗组对腹泻、脓血便、腹痛的缓解更具优势,治疗组总有效率(94.11%)高于对照组(76.47%),2组镜下表现积分均较前下降,组间比较差异有统计学意义。2组肠黏膜NF-κB mRNA的表达均较前下降,组间比较差异无统计学意义。结论:白头翁汤加减灌肠方较SASP能更好地缓解症状并改善肠黏膜的镜下表现,其作用机制可能通过下调NF-κB mRNA从而抑制促炎因子的产生及释放。Objectives To observe the clinical efficacy of Pulsatilla enema treatment on left colonic acute ulcerative colitis and observe the effect of it on NF-κB mRNA synthesis in mucosa. To discuss its possible mechanism. Method 34 cases were randomly divided into two groups. Treatment group were treated with the Pulsatilla retention enema while the cases of control group were treated with sulfasalazine retention enema. The total duration of treatment was both one and a half months. To make a comparison about the clinical efficacy between the two groups and observe the mucosa by colonoscopy. To measure NF-κB mRNA synthesis in mucosa by RT-PCR technique. Results All the symptoms improved in both groups after treatment. The symptoms of diarrhea,abdominal pain and mucopurulent bloody stool improved more significantly in the treatment group. The total effective rate was 94. 11% to 76. 47%. The treatment group＇s rate was higher. The treatment group＇s mucosal repaired better. The detected NF-κB mRNA quantity decreased significantly in both groups. But there was no statistical difference between the two groups. Conclusions Pulsatilla enema can relieve symptoms and repair intestinal mucosa better. The possible mechanism of Pulsatilla enema could be inhibiting NF-κB mRNA synthesis in mucosa,thus decreasing the producing and release of proinflammatory factors.

关 键 词：NF-ΚB MRNA 左半结肠型 急性期 中药灌肠 溃疡性结肠炎 

分 类 号：R285.5[医药卫生—中药学]