基于代谢网络技术的杜仲治疗皮肤光老化的靶向研究  

Study on the Treatment of Eucommia Ulmoides Oliver on Skin Photoaging based on Metabolic Network Analysis

作　　者：孙琪[1] 祁永华[1] 吴迪[1] 陈丹丹[2] 朴成玉[3] 石晨曦[1] 曹玲[4] 张宁[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [2]黑龙江中医药大学第二临床医学院,黑龙江哈尔滨150040 [3]黑龙江中医药大学药物安全性评价中心,黑龙江哈尔滨150040 [4]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《中国美容医学》2017年第8期70-73,共4页Chinese Journal of Aesthetic Medicine

基　　金：教育部"春晖计划"合作科研项目(编号:Z2010018)

摘　　要：目的:利用代谢网络技术研究杜仲治疗大鼠皮肤光老化的作用机制和作用靶点。方法:采用经典的皮肤光老化动物模型制备方法,参考临床生化指标对所建立的模型进行评价,判定模型组大鼠皮肤老化程度,采用无歧视的代谢网络分析技术对大鼠的尿液样本进行全局分析,聚焦关键代谢通路和作用靶点。结果:形态学考察发现其具有典型的皮肤光老化特征。皮肤组织的各项指标均显示模型组大鼠机体发生的显著地氧化受损过程,给予杜仲后出现一定回调趋势。尿液代谢轨迹分析发现杜仲具有显著回调皮肤光老化的作用。进一步聚焦分析发现皮肤光老化大鼠的代谢主要集中在花生四烯酸代谢和谷胱甘肽代谢通路,谷胱甘肽过氧化酶可能是其潜在的作用靶点。结论:杜仲对由紫外光照射诱导的皮肤光老化大鼠模型具有很好的回调作用,谷胱甘肽代谢是其涉及的核心代谢通路,谷胱甘肽过氧化酶为其潜在的作用靶点。Objective To study the mechanism and the target of Eucommia ulmoides Oliver on skin photoaging in rats by metabolic network analysis. Methods The classical animal model of skin photoaging preparation method, which conducted a certain dose of ultraviolet （UV） was induced for skin aging symptoms of sprague dawley rats. A series of clinical and biochemical indexes of oxidative damage were used for the degree of skin aging model judgements. At the same time, with non-discriminative network analysis technology, all the urine samples are conducted based on global analysis, which focused on the key metabolic pathways and targets. Results The animal model of skin photoaging was obtained by UV irradiation. The morphological characteristics of skin photoaging were typical All the indexes of the skin tissue showed significant oxidative damage in the body of the model group, and there was a certain callback trend. The analysis of urine metabolic track showed that the metabolic profiles of rats in the model group were significantly different from those of healthy rats, and there was a significant trend of callback in the two groups. Further analysis showed that the metabolism of skin photoaging rats was mainly concentrated in the metabolism of arachidonic acid and glutathione metabolism in the four. Conclusion Eucommia induced by ultraviolet irradiation on skin photoaging rat model has good effect on the callback, glutathione metabolism is the core metabolic pathway, glutathione peroxidase as potential targets.

关 键 词：杜仲 紫外光 光老化 代谢组学 谷胱甘肽 

分 类 号：R285.5[医药卫生—中药学]