水稻防御反应相关基因对稻瘟病菌的响应  

Response of Rice Defense-related Genes to Blast Fungus

作　　者：刘潮[1,2] 王慧[1] 刘林[1] 杨静[1] 李成云[1] 

机构地区：[1]云南农业大学/省部共建云南生物资源保护与利用国家重点实验室,昆明650201 [2]曲靖师范学院/云南高原生物资源保护与利用研究中心/云南省高校云贵高原动植物多样性及生态适应性进化重点实验室,曲靖655011

出　　处：《中国生物防治学报》2017年第4期504-511,共8页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2012CB722901)

摘　　要：水稻与稻瘟病菌的互作已成为研究植物与病原真菌互作的模式系统。利用RT-PCR技术检测了6个水稻品种(分别含有抗病基因Pik-s、Pita、Pit、Pi1、Pi9的近等系及回交亲本丽江新团黑谷LTH)与稻瘟病菌互作过程中多个信号相关及PR基因的表达。结果表明带有稻瘟病抗性基因Pik-s、Pita、Pit的水稻品种和LTH对稻瘟病菌#626侵染表现为亲和互作,带Pi1和Pi9的水稻品种表现为非亲和互作;稻瘟病菌接种后,亲和互作中MAPK6和MAPK12表现为上调表达,带有抗性基因Pi9的水稻品种IRBL22中BIMK2表现为上调表达。总体来看,含有不同抗病基因的水稻近等系中的PR基因对稻瘟病菌的响应较为多样,非亲和互作中在早期或早中期表现为PR基因上调表达,而亲和互作中主要在晚期上调表达,说明这些PR基因表达的时间在植物与病原互作的不同时期发挥着不同的作用。The rice–Magnaporthe oryzae pathosystem has become a model in the study of plant–fungal interactions. The expression of several signal associated and PR genes were analyzed in six rice lines（harboring resistance genes Pik-s, Pita, Pit, Pi1, Pi9 respectively with the backcross parent LTH） under M. oryzae infection by RT-PCR. The results showed that the rice lines harboring blast resistance gene Pik-s, Pita, Pit were compatible interaction with M. oryzae #626, the rice lines harboring Pi1 and Pi9 were incompatible interaction. The expression of MAPK12 and MAPK6 were up-regulated under susceptible interaction. BIMK2 showed higher expression under Pi9 mediated incompatible interaction. In conclusion, the PR genes expression pattern was more diversity to M. oryzae infection in harboring different resistance genes in rice monogenic lines. The up-regulation response of PR genes appeared in the early infection period in incompatible interactions, and at the late period in compatible interactions, indicating that the temporal expression pattern of these PR genes play an important role in plant pathogen interactions.

关 键 词：稻瘟病 防御相关基因 水稻近等基因系 互作 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]