娄彻氏链霉菌XL-6的抑菌活性及对茄子幼苗的防病促生效应  

The Antibiotic Activity of Fermentation Broth Produced from Streptomyces rochei XL-6 and Its Influence on Bacterial Wilt Control and Plant Growth in Eggplant Seedling

作　　者：李威[1] 肖熙鸥[1] 李可[1] 高晓敏[1] 吕玲玲[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所/湛江市热带作物遗传改良重点实验室,湛江524091

出　　处：《中国生物防治学报》2017年第4期531-536,共6页Chinese Journal of Biological Control

基　　金：中央级公益性科研院所基本科研业务费专项(1630062016004)

摘　　要：本文研究了娄彻氏链霉菌Streptomyces rochei XL-6无菌发酵液在自然状态下的抑菌活性及对茄子幼苗的防病促生效果。结果表明,菌株XL-6发酵液的抑菌活性物质可耐70℃高温,在pH 5~8其抑菌活性保持稳定;该抑菌活性物质不受紫外线、蛋白酶K和胰蛋白酶的影响,在4℃条件下贮藏28 d仍可保持良好的抑菌活性。采用不同浓度的发酵液处理茄子幼苗均可提高茄苗对青枯病的抗性,其中,施用40倍发酵液显著降低茄子青枯病发病率和病情指数并显著提高PAL和PPO酶活性,对盆栽茄子的防治效果达64.78%。同时,40倍发酵液显著提高茄子种子发芽率、鲜重、叶绿素含量及根系活力,较病原菌菌体处理和无菌水处理,茄苗干重增幅分别达15.81%和27.95%,对茄苗的促生效果显著。说明适宜浓度的拮抗菌XL-6发酵液兼具防治茄子青枯病和促进植株生长的效果。The antibiotic activity of fermentation broth produced from Streptomyces rochei strain XL-6 and its influence on bacterial wilt control and plant growth was analyzed. The result showed that the fermentation broth could still keep better antibiotic activity under 70 ℃ temperature, a wide range of p H range（5-8）, ultraviolet, proteinase K and trypin treatment, meanwhile, the fermentation broth could significant inhibit the Ralstonia solanacearum after stored 28 d under 4 ℃. The resistance to bacterial wilt in eggplant seedling was elevated by different dilution fermented, the 40-times dilution fermentation broth significantly decreased the bacterial wilt index and the control efficiency was 64.78%. The 40-times dilution fermentation broth not only elevated the PAL and PPO activity, but also increased the germination rate, fresh weight, chlorophyll content and activity of root. Compared with R. solanacearum and water treatment, the seedlings dry weight treated by 40-times dilution fermentation broth of XL-6 was increased 15.81% and 27.95% individual. In conclusion, the suitable dilution fermentation of XL-6 could control bacterial wilt occurrence in eggplant and promote plant growth.

关 键 词：拮抗菌 发酵液 稳定性 防治效果 促生作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]