深绿木霉蛋白质TraT2A诱导兰州百合抗灰霉病的作用  

Effect of Trichoderma atroviride Proteinaceous TraT2A Induced Lanzhou Lily Resistant to Gray Mold Caused by Botrytis cinerea

作　　者：梁巧兰[1] 张娜[1] 魏列新[1] 徐秉良[1] 

机构地区：[1]甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《中国生物防治学报》2017年第4期545-551,共7页Chinese Journal of Biological Control

基　　金：甘肃省财政厅项目(甘财教2013-116);甘肃省农牧厅生物技术专项(GNSW-2013-19;GNSW-2016-25)

摘　　要：采用生长速率法和活体试验法分别测定了深绿木霉Trichoderma atroviride蛋白质Tra T2A对兰州百合灰霉菌的抑制作用和诱导抗病效果及持效期。结果表明TraT2A高浓度(5×液)具有较高的抑制作用,抑制率为47.44%,低浓度(100×液)具有较高的诱导抗病作用,其诱导效果可达55.89%;TraT2A 100×液处理兰州百合植株3 d后挑战接种灰霉菌,分别于0、1、3和5 d对兰州百合叶片中的PAL、PPO、POD和SOD酶活性及丙二醛、叶绿素含量变化进行了测定。发现TraT2A诱导处理后可提高百合叶片中与抗病性相关的防御酶PAL、PPO、POD、SOD活性和叶绿素含量,降低丙二醛的含量;在接种后1、3和5 d时,4种酶活性和叶绿素含量显著高于对照,丙二醛含量则低于对照,4种酶活性在第5 d时均达到最大值,PAL、PPO、POD和SOD分别是对照的1.47、2.28、1.36和1.49倍;在接种后1、3和5 d时,叶绿素含量分别比对照高10.13、12.05和6.05 mg/g;丙二醛含量仅为对照的0.68、0.40和0.51倍;TraT2A防治百合灰霉病的持效期为7 d,高于阿泰灵和速克灵的持效期。The inhibition, induced resistance effect and persistence of TraT2A induced Lanzhou lily against gray mold caused by Botrytis cinerea were measured by growth rate method and in vivo test method. The results showed that the inhibition effects of high concentrations（5 times liquid） Trichoderma atroviride proteinaceous TraT2A was well, it was 47.44%, induced resistance effect to Botrytis cinerea of low concentration（100 times liquid） was higher, reached to 55.89%. Lily leaves were inoculated with Botrytis cinerea when lily were treated with 100 times liquid of TraT2A after three days, the enzyme activities of PAL, PPO, POD, SOD and MDA, chlorophyll content changes of lily leaves were measured in 0, 1, 3, 5 d of TraT2A inducing treatment. It was found that the activity of PAL, PPO, POD and SOD in the lily leaves was improved, and the content of chlorophyll was increased and the content of malondialdehyde was decreased after TraT2A induced treatment; the above four kinds of enzymes activity and chlorophyll content in lily leaves of TraT2A induced treatment were significantly higher than that of the control when inoculation 1, 3, 5 d, MDA content in lily leaves of TraT2A induced treatment was lower than that of the control, the activities of PAL, PPO, POD and SOD reached the maximum and which were 1.47, 2.28, 1.36, 1.49 times as those of the control in 5 d respectively; the content of chlorophyll were 10.13,12.05 and 6.05 mg/g higher than that of in the control; MDA content were only 0.68, 0.40, 0.51 times as that of the control after inoculation 1d, 3d and 5d. Persistence of TraT2A induced lanzhou lily against gray mold caused by Botrytis cinerea was 7 d and it was longer than persistence of 6% alternaria and 50% procymidone against B. cinerea.

关 键 词：深绿木霉蛋白质TraT2A 诱导抗病作用 机理 兰州百合 灰霉病 持效期 

分 类 号：S476[农业科学—农业昆虫与害虫防治]