运动训练改善PVN内前列腺素EP3受体介导的心衰大鼠交感神经激活作用  

Exercise Training Improves PTGER3 Receptor Mediated Sympathetic Outflow Within the Paraventricular Nucleus in Rats with Heart Failure

作　　者：王仁俊[1] 温美玲[1] 周琴[1] 李华[1] 未晓巍[1] 齐云峰[1] 栾剑[1] 徐洪伟[1] WANG Ren-jun WEN Mei-ling ZHOU Qin LI Hua WEI Xiao-wei QI Yun-feng LUAN Jian XU Hong-wei(College of Life Science, Jilin Normal University, Siping 136000, China)

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000

出　　处：《吉林师范大学学报（自然科学版）》2017年第3期106-111,共6页Journal of Jilin Normal University:Natural Science Edition

基　　金：国家自然科学基金项目(81202527);吉林省科技发展计划项目(20130522002JH);吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第212号)

摘　　要：为探讨运动改善心衰疾病的确切机制,采用心衰大鼠模型进行小动物跑台训练,检测PVN内微量注射PTGER3受体激动剂对交感驱动指标的影响,与假手术安静组相比,心衰安静组血浆中NE水平的升高和肾交感神经放电的增强作用发生敏感化(P<0.05).跑步运动训练明显改善心衰训练组大鼠交感驱动指标增强的敏感化作用(P<0.05).Western bolt实验结果显示:与假手术安静组相比,心衰安静组大鼠PVN内PTGER3受体蛋白和mRNA表达量发生上调(P<0.05).跑步运动训练明显下调心衰训练组大鼠PVN内PTGER3受体蛋白和mRNA表达量(P<0.05).结果提示运动训练可下调心衰大鼠PVN内PTGER3受体蛋白表达,进而抑制交感传出神经兴奋性.The goal of this study was to disclose the mechanism of exercise （ExT） in improving FFGER3 receptor mediated sympathetic outflow within the paraventricular nucleus （PVN） in rats with heart failure. Heart failure （HF） animal model was induced by coronary artery ligation and treadmill running was conducted. To examine the functional role of prostaglandin within the PVN, an agonist of PTGER3 receptor, sc- 46275 ,was microinjected into the PVN. Dose-dependent increases in plasma norepinephrine （NE）, RSNA, arterial blood pressure （BP） ,and heart rate （HR） were produced in all rats. There was a significantly increase in these parameters in HF rats compared with the sham-operated rats. However, ExT significantly improved the sensitive phemomenon in HF rats. The results of western blot show that PTGER3 receptor mRNA expression levels in the PVN are also significantly increased in HF-ExT rats compared with HF-sedentary rats. Our results indicate that ExT improves PTGER3 receptor mediated sympathetic outflow within the paraventricular nucleus in rats with heart failure and the ＂mechanism may be involved in the downregulation of PTGER3 receptor in PVN.

关 键 词：心衰 运动训练 室旁核 前列腺素 受体 

分 类 号：R541.61[医药卫生—心血管疾病] R541.62[医药卫生—内科学]