Cytokine Array蛋白点表达丰度分析软件的优选研究  

Preferentially selecting the image analysis software for the abundance analysis of the proteinic spots of the Cytokine Array

作　　者：黄金浩 高闯[1] 张志飞[1] 苏万强[1] 王鹏[1] 魏文桂[1] 孙健[1] 江荣才[1] 

机构地区：[1]天津医科大学总医院神经外科,300052

出　　处：《中华实验外科杂志》2017年第8期1299-1302,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81271359）;天津市科技计划项目（14ZCZDSY00179）

摘　　要：目的 比较3款常用图像分析软件对细胞因子矩阵（Cytokine Array）相同任意蛋白点表达的差异,优选出最适图像分析软件.方法 从本实验室获得的一对Cytokine Array图像,任选图上4个蛋白点,以3款图像分析软件（Gel-Pro analyzer/Labwork、Quantity One和Image J）反复分析其蛋白丰度,比较其复杂性和可重复性,并针对入选蛋白,行酶联免疫吸附试验（ELISA）对比验证其吻合程度.结果 3款分析软件分析过程的复杂程度由简到繁依次为Gel-Pro analyzer/Labwork、Quantity One和Image J;可重复性从大到小（即变异系数从小到大）依次为Gel-Pro analyzer/Labwork（0.00%）、Quantity One（5.07%）和Image J（9.75%）;若以ELISA（81.49%）结果为标准参考值,则吻合度从高到低依次为Quantity One（71.61%）、Image J（67.27%）、Gel-Pro analyzer/Labwork（62.31%）.结论 各种软件均具备独自特点,如果仅作定性分析,推荐Gel-Pro analyzer/Labwork;如果作半定量分析,推荐Quantity One.若综合可重复性、分析过程的复杂程度、与ELISA结果的吻合程度,则推荐Quantity One.Objective In order to preferentially select the most suitable image analysis software for the abundance analysis of the proteinic spot of Cytokine Array,we compared 3 kinds of image analysis software in analyzing the randomly selected proteinic spots of the Cytokine Array.Methods Four proteinic spots were randomly selected from a pair of Cytokine Array images obtained in our lab.Three image analysis softwares of Gel-Pro analyzer/Labwork,Quantity One and Image J were applied to repeatedly analyze the abundance of the selected proteinic spots.The repeatability and complexity of the analysis procedures of these softwares were assayed.In order to test whether the software analysis result agreed to that of the enzyme linked immunosorbent assay （ELISA）,the abundance of the corresponding proteins of the selected proteinic spots were also assayed with ELISA.Results The highest and lowest repeatability of the analysis result of the software ranked as Gel-Pro analyzer/Labwork,Quantity One and Image J.The least and the most procedure complexity of the software was Gel-Pro analyzer/Labwork,Quantity One and Image J.The best to the poorest agreement degrees of the software analysis results with the ELISA assay ones were Quantity One,Image J and Gel-Pro analyzer/Labwork.Conclusion In the proteinic spots evaluation in Cytokine Array analysis,the Gel-Pro analyzer/Labwork is suggested for qualitative analysis and the Quantity One is recommended for semi-quantitative analysis.If taking into account the repeatility,complexity and agreement to ELISA,the Quantity One is proposed first.

关 键 词：图像分析软件 细胞因子矩阵 灰度值 

分 类 号：R651.15[医药卫生—外科学] TP391.41[医药卫生—临床医学]