焦炉工尿中多环芳烃羟基代谢产物影响因素分析  

Influencing factors of urinary hydroxyl metabolites of polycyclic aromatic hydrocarbons in coke oven workers

作　　者：孟潇[1] 段化伟[2] 刘仲[3] 张志虎[1] 

机构地区：[1]山东省职业卫生与职业病防治研究院,山东济南250062 [2]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [3]济南市疾病预防控制中心,250021

出　　处：《中国职业医学》2017年第4期425-429,共5页China Occupational Medicine

基　　金：国家科技支撑计划项目(2014BAI12B02);国家自然科学基金(81172642)

摘　　要：目的观察焦炉工尿中4种多环芳烃(PAHs)羟基代谢产物水平及其影响因素,探讨其作为焦炉逸散物(COEs)接触的生物标志物的可行性。方法采用横断面调查方法,以某焦化厂261名焦炉工为接触组,以某氧气厂111名非焦炉作业工人为对照组。采用超高液相色谱-质谱法检测2组人群尿中1-羟基芘、1-羟基萘、2-羟基萘和3-羟基菲4种PAHs羟基代谢产物的水平。结果接触组人群尿中4种PAHs羟基代谢产物水平均高于对照组(P<0.01);接触组3个亚组人群尿中1-羟基芘、1-羟基萘和2-羟基萘水平从低到高依次为炉底亚组<炉侧亚组<炉顶亚组(P<0.05)。多重逐步回归分析结果显示:在排除性别、年龄、吸烟和饮酒等混杂因素的影响后,尿中1-羟基芘、1-羟基萘和2-羟基萘水平均与接触COEs有关联(P<0.05);在排除性别、年龄、工龄、吸烟和饮酒等混杂因素的影响后,接触组人群尿中1-羟基芘、1-羟基萘和2-羟基萘水平均呈随COEs接触水平增加而增加的剂量-效应关系(P<0.01);接触组人群尿中1-羟基萘和2-羟基萘水平还与吸烟有关联(P<0.01)。结论 1-羟基芘可作为COEs内暴露评价的可靠生物标志物,1-羟基萘和2-羟基萘可作为COEs内暴露评价的辅助生物标志物。Objective To observe the levels of four hydroxylated metabolites of polycyclic aromatic hydrocarbons （PAHs） in the urine of coke workers and its influencing factors. To explore the feasibility of using PAHs as biomarkers for exposure of coke oven emissions （COEs）. Methods A cross-sectional survey was used to compare 261 coke oven workers in a coke oven plant as exposure group with 111 workers without COEs exposure in an oxygen making plant as control group. Ultra high liquid chromatography-mass spectrometry was used to detect four hydroxylated metabolites of PAHs, including 1-hydroxypyrene （OHP）, I-hydroxynaphthalene （OHN）, 2-OHN and 3-bydroxyphenanthrene, in urine of these two groups. Results The levels of four hydroxylated metabolites of PAHs in urine in exposure group were higher than that of the control group （P 〈 0.01 ）. The levels of urinary 1-OHP, 1-OHN, 2-OHN were followed by the sequence of bottom- oven, side-oven, and top-oven subgroups among the exposure group （ P 〈 0. 05 ）. The multiple linear regression results indicated that the levels of urinary 1-OHP, 1-OHN, 2-OHN were correlated with COEs exposure （ P 〈 0. 05 ） , after adjusting the confounding factors of gender, age, length of service, smoking status and alcohol drinking status. The levels of urinary 1-OHP, 1-OHN, 2-OHN of the exposure group increased with the increase of COEs exposure levels showing a dose-effect relationship （ P 〈 0.01 ）. The levels of 1 -OHN and 2-OHN were associated with smoking apart from COEs exposure （ P 〈 0. 01 ）. Conclusion The urinary 1-OHP can be used as a reliable biomarker for the evaluation of internal exposure to COEs. The 1-OHN and 2-OHN can be used as adjuvant biomarkers.

关 键 词：焦炉逸散物 多环芳烃 生物标志物 羟基代谢产物 1-羟基芘 1-羟基萘 2-羟基萘 3-羟基菲 

分 类 号：R135[医药卫生—劳动卫生]