TNF-α release capacity is suppressed immediately after hemorrhage andresuscitation  

作　　者：Arian Bahrami Mohammad Jafarmadar Heinz Redl Soheyl Bahrami Jian-Xin Jiang 

机构地区：[1]Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in AUVA Research Center, 1200 Vienna, Austria [2]State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Doping Hospital, Third Military Medical University, Chongqing, China

出　　处：《Chinese Journal of Traumatology》2017年第4期207-211,共5页中华创伤杂志（英文版）

摘　　要：Purpose： It has been suggested that patients with traumatic insults are resuscitated into a state of an early systemic inflammatory response. We aimed to evaluate the influence of hemorrhagic shock and resuscitation （HSR） upon the inflammatory response capacity assessed by overall TNF-α secretion capacity of the host compared to its release from circulating leukocytes in peripheral circulation. Methods： Rats （8/group） subjected to HS （MAP of 30e35 mmHg for 90 min followed by resuscitation over 50 min） were challenged with Lipopolysaccharide （LPS）, 1 mg/kg intravenously at the end of resuscitation （HSR-LPS group） or 24 h later （HSR-LPS24 group）. Control animals were injected with LPS without bleeding （LPS group）. Plasma TNF-α was measured at 90 min after the LPS challenge. In addition, whole blood （WB） was obtained either from healthy controls （CON） immediately after resuscitation （HSR）, or at 24 h post-shock （HSR 24）. WB was incubated with LPS （100 ng/mL） for 2 h at 37 C. TNF-α concentration and LPS binding capacity （LBC） was determined. Results： Compared to LPS group, HSR followed by LPS challenge resulted in suppression of plasma TNF-a in HSR-LPS and HSR-LPS24 groups （1835 ± 478, 273 ± 77, 498 ± 200 pg/mL, respectively）. Compared to CON the LPS-induced TNF-a release capacity of circulating leukocytes ex vivo was strongly declined both at the end of resuscitation （HSR） and 24 h later （HSR24） （1012 ± 259, 313 ± 154, 177 ± 63 ng TNF/mL, respectively）. The LBC in WB was similar between CON and HSR and only moderately enhanced in HSR24 （57 ± 6, 56 ± 6, 71 ± 5 %, respectively）. Conclusion： Our data suggest that the overall inflammatory response capacity is decreased immediately after HSR, persisting up to 24 h, and is independent of LBC.

关 键 词：INJURIES Endotoxins shock IMMUNOSUPPRESSION 

分 类 号：R641[医药卫生—外科学]