天麻灵芝颗粒药效学实验研究  

Pharmacodynamic study on Tianma Lingzhi Granules

作　　者：吴静澜[1] 刘明[1] 刘莉 罗战彪 王海洋 张永萍[1] 

机构地区：[1]贵阳中医学院药学院,贵州贵阳550025 [2]贵阳德昌祥药业有限公司,贵州贵阳550081

出　　处：《中草药》2017年第16期3440-3446,共7页Chinese Traditional and Herbal Drugs

基　　金：天麻医药产业深度开发关键问题研究(黔科合重大专项字[2011]6005);贵州省普通高等学校中药民族药(苗药)新剂型新制剂工程研究中心(黔教合KY字[2014]22号);贵州省中药民族药炮制与制剂工程技术研究中心(黔科合重G字[2012]4001号);贵州省中药(民族药)药剂学专业学位研究生工作站(黔教研合YJSZ字[2013]05号);贵州省现代民族药(苗药)协同创新中心(黔教合协同创新字[2013]02号)

摘　　要：目的对天麻灵芝颗粒进行药效学研究。方法通过小鼠断头张口喘气实验、耐常压缺氧实验、豚鼠氯仿所致眩晕实验、旋转刺激缺血性眩晕模型大鼠水迷宫实验,考察天麻灵芝颗粒抗眩晕作用;通过小鼠自主活动实验、小鼠协同戊巴比妥钠催眠实验,考察天麻灵芝颗粒的镇静催眠作用;通过小鼠扭体法实验、小鼠热板法实验,考察天麻灵芝颗粒镇痛作用;通过游泳和调节免疫实验,考察天麻灵芝颗粒抗疲劳及调节免疫的作用。结果天麻灵芝颗粒能促进大脑供血供氧;能明显减少小鼠的自主活动次数,增加阈下剂量戊巴比妥钠睡眠小鼠只数,缩短阈剂量戊巴比妥钠小鼠入睡潜伏期并延长睡眠时间;能显著地增加醋酸致小鼠扭体反应潜伏期并减少扭体反应次数,增加热板法小鼠痛阈值;能明显延长小鼠游泳时间,增加幼龄小鼠脾脏、胸腺指数,增加小鼠的碳粒廓清指数和吞噬指数。结论天麻灵芝颗粒具有抗眩晕、镇静催眠、镇痛、抗疲劳及提高免疫功能的作用。Objective To study the pharmacodynamics of Tianma Lingzhi Granules（TLG）. Methods Mouth breathing experiment and hypoxia tolerance experiment under atmospheric pressure with mice, experiment of dizziness of guinea pigs caused by chloroform, and stimulus ischemic vertigo of model rats caused by rotating in water maze experiment was used to investigate the anti-vertigo effect of TLG; The experiment of autonomic activities of mice and hypnosis experiment of mice with pentobarbital sodium was used to investigate the sedative hypnotic effect of TLG; The experiments of torsion body and hot plate of mice was used to investigate the analgesic effect of TLG; The experiments of swimming and regulating immune experiment was used to investigate the anti-fatigue and immune regulation effect of TLG. Results TLG could promote the supplying of oxygen in the brain, significantly reduce the times of autonomic activities of mice, increase the number of sleeping mice at the subthreshold dose of sodium pentobarbital, shorten the sleep latency of mice with hreshold dose of sodium pentobarbital and prolong the sleep time, significantly increase mice body torsion reaction latency by the acetic acid and reduce the body torsion times, and increase the spleen thymus index, clearance coefficient and phagocytic index of mice. Conclusion TLG has the effect of treating dizziness, forgetfulness, waist and knee fatigue weakness and so on.

关 键 词：天麻灵芝颗粒 抗眩晕 镇静催眠 镇痛 抗疲劳 调节免疫 

分 类 号：R285.5[医药卫生—中药学]