基于灰色关联度分析的丹参提取物抗炎作用谱效关系研究  

Spectra-effect relationship of anti-inflammation of Salvia miltiorrhiza extract based on gray correlation analysis

作　　者：林梦雅[1] 张玉萍[1] 李雅[1] 姜民[2] 庄朋伟[1] 张艳军[1] 宋丽丽[1] 

机构地区：[1]天津中医药大学中药学院,天津300193 [2]南开大学药学院,天津300071

出　　处：《中草药》2017年第16期3447-3452,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划项目(2011BAI07B08)

摘　　要：目的研究丹参不同提取物的超高效液相色谱(UPLC)图谱与抗炎作用的谱效关系,为明确丹参抗炎作用物质基础提供依据。方法采用UPLC法对丹参不同提取部位(S1~S9)进行分析,以人支气管上皮细胞炎症模型考察不同提取部位的抗炎作用,采用灰色关联度分析法建立其谱效关系。结果丹参醇提物50%乙醇洗脱液(S3)、80%乙醇洗脱液(S4)、滤过沉淀(S5)以及萃取后水层提取物(S9)对于炎症细胞抗炎能力强,其中以丹参醇提物的50%乙醇洗脱液(S3)作用能力最强。丹参发挥抗炎作用的药效是多种成分共同作用的结果,各特征峰所代表的化学成分对其抗炎药效贡献的大小顺序为9号峰>1号峰>2号峰>6号峰>15号峰>11号峰>12号峰>7号峰>10号峰>5号峰>14号峰>3号峰>18号峰>4号峰>13号峰>16号峰>8号峰,对抗炎作用药效贡献大的前4个峰分别为9、1、2、6号峰,经过与对照品比对及质谱分析鉴定出4个峰对应的成分分别为丹参酮IIA、丹参素、丹酚酸B和3′-甲基丹酚酸B。结论丹参抗炎作用的药效是多种成分共同作用的结果,明确了丹参中丹参酮IIA、丹参素、丹酚酸B和3′-甲基丹酚酸B这4个成分对其抗炎作用的药效贡献最大,为今后探索丹参药材抗炎作用的药效物质基础提供了一种新思路。Objective To study the relationship between UPLC and anti-inflammatory effects of different extracts of Salvia miltiorrhiza, and to provide a basis for clarifying the material basis of anti-inflammatory effects of S. miltiorrhiza. Methods UPLC was used to analyze the different extraction sites of S. miltiorrhiza. The anti-inflammatory effect of anti-bronchial epithelial cell inflammation model was studied by different extraction sites. The spectral relationship was established by gravitational correlation analysis（GRA）. Results 50% grams of ethanol extract（S3）, 80% ethanol eluent（S4）, filtered precipitate（S5）, and extracted aqueous layer extract（S9） had strong anti-inflammatory ability to inflammatory cells. The 50% ethanol eluate（S3） of the ethanol extract of Danshen was the strongest. The effect of chemical composition on the anti-inflammatory efficacy of each characteristic peak was as the following order： A9〉A1〉A2〉A6〉A15〉A11〉A12〉A7〉A10〉A5〉A14〉A3〉A18〉A4〉A13〉A16〉A8, and the top four peaks with strong contribution to anti-inflammatory effects were peaks 9, 1, 2, and 6, respectively. The results were as follows： tanshinone IIA, danshensu, salvianolic acid B, and 3＇-methyl salvianolic acid B. Conclusion The anti-inflammatory efficacy of S. miltiorrhiza is the result of combination of various components. It is clear that the four components are tanshinone IIA, danshensu, salvianolic acid B, and 3＇-methyl salvianolic acid B. The efficacy of the largest contribution to the future to explore the efficacy of anti-inflammatory effects of S. miltiorrhiza provides new ideas.

关 键 词：丹参 抗炎作用 灰色关联度分析 谱效关系 丹参酮IIA 丹参素 丹酚酸B 3′-甲基丹酚酸B 

分 类 号：R286.02[医药卫生—中药学]