丁香酚、小檗碱及苦参碱对荔枝霜疫霉的抑制作用  

Inhibitory effects of eugenol,berberine and matrine on Peronophythora litchi

作　　者：曾令达[1,2] 陆飞莹 宋冠华[1,2] 曾海泉[3] 

机构地区：[1]惠州学院生命科学学院,广东惠州516007 [2]惠州学院生物技术研究所,广东惠州516007 [3]惠州市农业科学研究所,广东惠州516023

出　　处：《广东农业科学》2017年第5期106-111,共6页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2013B020304010);惠州市科技计划项目(2009B010001024);惠州学院自然科学项目(C211.0220)

摘　　要：为了筛选防治荔枝霜疫霉(Peronophythora litchi Chen)的植物源抑菌活性物质,以丁香酚、小檗碱及苦参碱3种植物源抑菌活性物质为材料,采用菌丝生长速率法测定3种材料抑制荔枝霜疫霉效果;霜疫霉孢子囊接种荔枝果实后测定3种物质在荔枝贮藏中的抑菌效果。结果表明,丁香酚、小檗碱、苦参碱3种植物提取物对荔枝霜疫霉均具有抑菌活性,其中丁香酚的毒力方程为y=1.5909x+9.6688,抑菌中浓度(EC50)为1.16μg/m L;小檗碱的毒力方程为y=1.2129x+6.6709,EC50为42.03μg/m L;苦参碱的毒力方程为y=1.6217x+6.5849,EC50为105.01μg/m L;丁香酚处理接种霜疫霉的果实褐变指数为96.9%,病情指数为100.0%,小檗碱和苦参碱处理褐变指数与对照差异不显著,但病情指数分别为68.4%和75.3%,与对照差异显著。3种物质中丁香酚的EC50最小,离体抑菌效果最强,其次为小檗碱和苦参碱;果实抑菌效果中最佳为小檗碱,其次为苦参碱和丁香酚。In order to find botanical antifungal substances that can prevent Peronophythora litchi Chen, using the myeelial growth rate in vitro euhure inhibition test, the antifungal effects of eugenol, matrine and berberine were measured after inoculation of litehi fruit in storage. The results show that three plant extracts had inhibitory effects on Peronophythora. The toxicity regression equation of eugenol was y =1.5909x＋9.6688, the EC5o was 1.16 I~ g/mL. The toxicity regression equation of berberine was y =1.2129x＋6.6709, the ECs0 was 42.03 I.L g/mL. The toxicity regression equation of matrine was y--1.6217x＋6.5849, the ECs0 was 105.06 I~ g/mL. The browning index of fruit inoculated Phytophthora treated with eugenol was 96.9%, and the disease index was 100.0%. Compared with the control, the browning index of berberine and matrine was not obvious, hut the disease indexes were 68.4% and 75.3%, which were significantly different from that of the control. Among the three plant extracts, eugenol had the best inhibitory effect, next one was berberine and the worst was matrine. For inhibitory effect of fruit, the best was berberine, followed by matrine and eugenol.

关 键 词：丁香酚 小檗碱 苦参碱 荔枝霜疫霉 抑菌效果 

分 类 号：S482.292[农业科学—农药学]