不同产地马兜铃蜜炙前后HPLC指纹图谱分析  

HPLC Fingerprint of Fructus Aristolochiae and Honey Processed Products from Different Habitats

作　　者：章莹[1,2,3] 肖榕[1,2,3] 黄杰[1,2,3] 吴萍[1,2,3] 林丽美[1,2,3] 夏伯侯[1,2,3] 廖端芳[1,2,3] 

机构地区：[1]湖南中医药大学药学院,长沙410208 [2]中药有毒物质防控技术湖南省工程实验室,长沙410208 [3]湘产大宗药材品质评价湖南省重点实验室,长沙410208

出　　处：《中国药学杂志》2017年第16期1397-1402,共6页Chinese Pharmaceutical Journal

基　　金：马兜铃的饮片炮制规范研究(2015-YP-31);长沙市科技计划重点项目(k1406030-31);湖南省教育厅重点项目(14A109);湖南中医药大学研究生科研创新项目(2015CX19)

摘　　要：目的建立不同产地马兜铃蜜炙前后的HPLC指纹图谱,比较分析马兜铃蜜炙前后化学成分变化。方法采用高效液相色谱分析,Agilent ZORBAX SB-C_(18)柱(4.6 mm×250 mm,5μm),流动相为乙腈-1%乙酸溶液,梯度洗脱,流速1.0 mL·min^(-1),检测波长250 nm,柱温30℃;采用《中药色谱指纹图谱相似度评价软件》、SPSS22.0软件对HPLC色谱进行相似度分析、聚类分析及主成分分析。结果建立14批不同产地马兜铃蜜炙前后的指纹图谱,蜜炙后马兜铃酸Ⅰ(AAⅠ)降低率达36%以上;相似度评价显示生、制马兜铃相似度大于0.9(除S4为0.822,S18为0.771,S19为0.639);聚类分析表明,生品马兜铃大致分为4类,主成分分析可知,5个主成分(包括AA I)的累积方差贡献率为84.914%,从化学成分上反映了不同产地样品的相似性及差异性。结论本实验结果可以为马兜铃炮制减毒及炮制工艺的规范化提供参考,具有示范性。OBJECTIVE To establish the HPLC fingerprints of Fructus Aristolochiae and honey-fried products from different habitats,compare and analyze their difference in chemical components. METHODS Using an Agilent ZORBAX SB-C18 column（ 4. 6 mm × 250 mm,5 μm）,HPLC method was performed to collect the fingerprints. The mobile phase A and B were acetonitrile and 1% acetic acid,respectively. Gradient elution was performed at the flow rate of 1. 0 mL ·min-1 and column temperature of 30 ℃. The detection was carried out at 250 nm. Similarity Evaluation System for Chromatographic Fingerprint of TCMs（ Ver. 2012） and SPSS22. 0 was used in cluster analysis and principal component analysis（ PCA）. RESULTS The fingerprints of 14 batches of Fructus Aristolochiae samples,before and after process,were established by HPLC. The reduction rate of AAⅠ,a poisonous component,was over 36%. Except for S4（ 0. 822）,S18（ 0. 771） and S19（ 0. 639）,the similarities of Fructus Aristolochiae and processed products were over 0. 9. The non-processing samples were classified into four groups by cluster analysis. As PCA shown,the five principal components including AAⅠ had a contributing rate of 84. 914% to cumulative sum of squares,illustrating the chemical similarity and difference of samples from different areas. CONCLUSION The results may provide reference for Fructus Aristolochiae processing and standardization of its detoxification technology.

关 键 词：马兜铃 马兜铃酸Ⅰ 高效液相色谱法 指纹图谱 聚类分析 主成分分析 

分 类 号：R282[医药卫生—中药学]